人原发性肝细胞癌地高辛素标记HBV DNA探针的原位杂交与C－myc和P_（53）癌基因表达相互关系的研究

取自肝癌高发区的４０例人原发性肝细胞癌（ＰＨＣ）标本，应用地高辛素（Ｄｉｇ）标记ＨＢＶＤＮＡ探针作原位杂交，检测ＰＨＣ和癌周肝组织内ＨＢＶＤＮＡ的表达，并用光敏生物素Ｃ－ｍｙｃ癌基因探针原位杂交和用免疫组化检测ＨＢｓＡｇ，ＨＢｃＡｇ及Ｐ５３癌基因在ＰＨＣ和癌周肝组织内的表达。Ｄｉｇ－ＨＢＶＤＮＡ探针肝内总阳性率为８０％，在ＰＨＣ内为６５％，而癌周肝组织中为７３％。ＨＢＶ－ＤＮＡ定位于肝细胞或癌细胞的胞浆和胞核内，而ＨＢＶＤＮＡ阳性颗粒在胞浆内比胞核内多。ＨＢＶＤＮＡ阳性细胞癌周比癌内更多。结果表明，ＰＨＣ病例与ＨＢＶ存在关系密切；癌内ＨＢＶＤＮＡ大都属整合型，而癌周属游离型。研讨ＰＨＣ内ＨＢＶＤＮＡ的表达与ＨＢｓＡｇ，ＨＢｃＡｇ，和Ｐ５３及Ｃ－ｍｙｃ癌基因的表达相互关系及致癌作用。

A STUDY OF HBV DNA IN HUMAN PHC BY SITU HYBRIDIZATION USING A DIGOXIGENIN LABELLED PROBES AND EXPRESSION ASSOCIATED WITH C-myc AND P_(53) ONCGENE

Surgical specimens of 40 patients with human primary hepatocellular carcinoma(PHC) from the high-liver-cancer-incidence region were studied by in situ hybridization with Digoxigenin labelled probe for detecting HBV DNA and with photobiotin labelled probe for detecting expression of C-myc oncgene and by immunohistochemical staining for expression of HBsAg HBcAg and P53gene in PHC and pericancerous liver tissue respectively on formalin-fixed parafin-embedded section. The positive rate of HBV DNA was totally 80%(38/40)in the liver tissue, with 65%(26/40) in PHC tissuc and 73%(8/11) in the pericancerous liver tissue. HBV DNA was found mainly in cytoplasm of hepatocytes and cancer cells and more in the nucleus. More HBV DNA gunules were seen in the cytoplasmlsm than in nucleus. More HBV DNA positive cells were found in the pericancerous tissue than in cancerous tissue. These results showed that most of PHC cases selected were closey associated with the infection of HBV,HBV DNA in the pcricancerous tissue might be of the dissociate form, whie in the cancerous tissue it might be of the integrated form. The Expressive relationship between HBV DNA and HBsAg, UBcAg and C-myc gene,P53 gene in the liver and their pathogenesis are disscussed in this paper.