重楼对多发性创伤大鼠急性肺损伤的保护作用

目的:探讨重楼总皂苷(RPTS)对多发性创伤模型大鼠急性肺损伤(ALI)的影响。方法:将36只Wistar大鼠随机分成3组,除空白对照组外,其余两组按多发骨折-脂多糖两次打击致多发性创伤并发急性肺损伤动物模型标准制模,制模后1h,重楼干预组予重楼总皂苷(RPTS)灌胃。干预后6h,采血以ELISA法检测血清中TNF-α、IL-1β及IL-6浓度;各组留取肺组织,光镜下进行病理形态观察。结果:重楼干预组大鼠血清TNF-α、IL-1β及IL-6浓度显著低于模型对照组(P<0.001)。模型对照组,光镜下肺组织病理形态观察显示明显肺损伤;而重楼干预组肺损伤表现明显轻于模型对照组。结论:重楼总皂苷对多发性创伤大鼠急性肺损伤具有保护作用,其机制可能与下调血清中的TNF-α、IL-1β、IL-6水平从而减轻炎症反应有关。

Protective effects of rhizoma paridis total saponins on acute lung injury rats subjected to multiple trauma

Objective:To investigate the protective effects of rhizoma paridis total saponins(RPTS)on acute lung injury rats subjected to multiple trauma.Methods: Thirty-six Wistar rats were randomly divided into three groups, and were made to the model in accordance with the standard of two-hit animal model induced by multiple fractures and lipopolysaccharide ,except the blank control group. 1 hour later,rats in RPTS groups were given Rhizoma Paridis total saponins by intragastric administration. 6 hours later,the concentrations of TNF-α、IL-1β and IL-6 in the blood serum of all rats were detected by ELISA(enzyme linked immunosorbent assay). The pathological changes of the lung tissues were examined by light microscope.Results: In RPTS groups ,the concentrations of TNF-α、IL-1β and IL-6 in blood serum of rats were remarkably lower than those in model group(P<0.001). In the model group, the evidence of lung injury was discovered by light microscopy. In RPTS groups,the histological changes of lung injury were ameliorated. Conclusion: Rhizoma paridis total saponins can protect acute lung injury rats subjected to two-hit by falling the concentrations of TNF-α、IL-1β and IL-6 in blood serum of rats and inhibiting the lung inflammatory injury.