褪黑素对NB4细胞作用的研究

目的观察褪黑素对人早幼粒细胞白血病细胞株NB4凋亡、分化及细胞周期的影响。方法以不同浓度的褪黑素(0.1、0.5、1mmol/L)加入NB4细胞中,继续培养24、48、72h,利用流式细胞术检测细胞凋亡率,细胞膜表面抗原CD33和CD11b的表达阳性率,及细胞周期的变化情况。结果MLT有促进NB4细胞凋亡的作用,且呈时间和浓度依赖性增加(P<0.05);CD33的细胞阳性率没有变化(P>0.05),CD11b的细胞阳性率在浓度为1mmol/L,48h和72h有轻度增加(P<0.05);细胞周期检测则显示随着浓度和时间的增加,G1期细胞增多,S期细胞减少(P<0.05)。结论褪黑素有促进NB4细胞凋亡,影响细胞生长周期的作用,诱导分化作用则不明显。

Study on the effects of melatonin on NB4 cells

Objective To investigate the effects of melatonin on NB4 cells.Methods The NB4 cells were treated with melatonin of different concentration(0.1,0.5,1 mmol/L) for different duration(24,48 and 72 h) in culture.The apoptosis,expression of CD11b and CD33,and cell cycle were detected by flow cytometry.Results Melatonin induced apoptosis of NB4 cells in a dose-and-time-dependent manner(P<0.05).The expression of CD33 was not different among different groups,while CD11b increasesd in group of 1 mmol/L treated for 48 and 72 h.FCM analysis also showed that the percentage of cells in G1-phase increased(P<0.05).Conclusion Melatonin increases apoptosis and inhibits progression from G1 to S phase of the cell cycle,but is not obvious different on NB4 cells.