Peroxiredoxin 1与EMT相关蛋白在胰腺癌组织中的表达及其临床意义

目的：探讨过氧化物还原酶1( Prx1)与上皮细胞间质转化( EMT)相关蛋白在胰腺癌组织中的表达及其临床意义。方法采用免疫组化法分别检测 Prx1、E-钙黏素( E-cadherin)、波形蛋白( Vimentin)在66例胰腺癌组织和11例正常胰腺组织中的表达,并分析三者之间的关系及与临床病理因素间的关系。结果 Prx1、E-cadherin和Vimentin分别在66例胰腺癌组织中的阳性表达率为69．70%(46/66)、40．91%(27/66)、56．06%(37/66)；Prx1、E-cadherin和Vim-entin分别在11例正常胰腺组织中的阳性表达率为18．18%(2/11)、90．90%(10/11)、18．18%(2/11)。 Prx1、E-cadherin和Vimentin在胰腺癌组织中的表达均与TNM分期、淋巴结转移和肿瘤分化程度显著相关(P<0．05),而与性别、年龄、肿瘤大小、病变部位均无关；Prx1与神经侵犯显著相关( P<0．05),而E-cadherin和Vimentin与神经侵犯无关。 Prx1的表达与Vimentin表达呈正相关(P<0．05),Prx1和Vimentin的表达均与 E-cadherin表达呈负相关( P<0．05)。结论Prx1与EMT相关蛋白在胰腺癌组织中的表达呈显著相关性,并与其侵袭转移能力相关,提示Prx1的高表达可能为预测胰腺癌患者预后不良的辅助指标之一。

Clinical significance of expression of Peroxiredoxin 1 and EMT related factors in pancreatic carcinoma

Objective To investigate the expression clinical significance of Peroxiredoxin 1 ( Prx1 ) and epithelial mesenchymal transition (EMT)-related factors in pancreatic carcinoma. Methods The expression of Prx1, E-cad-herind and Vimentin were detected in 66 pancreatic carcinoma samples and 11 normal pancreatic tissues by immu-nohistochemical method. The correlation between Prx1, E-cadherin, Vimentin and clinicopathologic characteristics was also analyzed. Results The positive expression rates of Prx1, E-cadherind and Vimentin were 69. 70% (46/66), 40. 91% (27/66) and 56. 06% (37/66) in pancreatic carcinoma, and 18. 18% (2/11), 90. 90% (10/11) and 18. 18% (2/11) in normal pancreatic tissue, respectively. The expression rates of Prx1, E-cadherin and Vimentin were correlated with TNM stage, lymph node metastasis, degree of differentiation ( P <0. 05 ) . Prx1 expression was correlated with nerve invasion in pancreatic carcinoma tissue ( P<0. 05 ) . The expression rates of
Prx1, E-cadherin and Vimentin were not correlated with sex, age, size, site of tumor. E-cadherin and Vimentin expression were not correlated with nerve invasion inpancreatic carcinoma tissue. Prx1 expression in pancreatic car-cinoma was positively correlated with Vimentin expression ( P<0. 05 );Prx1 and Vimentin were negatively correla-ted with E-cadherin expression ( P<0. 05 ) . Conclusion Prx1 , E-cadherin and Vimentin expressions are associ-ated with the ability of invasion and metastasis in pancreatic carcinoma, Prx1 abnormal expression maybe serve as a potential poor prognostic marker of patients with pancreatic carcinoma.