TP-ELISA、TPPA法检测梅毒抗体的结果分析

目的 比较TP-ELISA和TPPA方法检测血清中梅毒抗体阳性率的检测差异。方法 对4000例血清标本分别用TP-ELISA、TPPA进行梅毒抗体阳性率检测。结果 TP-ELISA法阳性率为3.60%(144/4000),TPPA法阳性率3.45%(138/4000),阳性符合率为92.36%(133/144),两种方法对梅毒感染的检出率尚不能认为存在差异(χ²=0.132,P＞0.05)。结论 在临床检验中,TP-ELISA可作为梅毒的筛查试验,阳性结果 再进一步作TPPA法确诊,以减少梅毒的误诊和漏诊。

Clinical value of combined detection of TP-ELISA and TPPA in diagnosis to syphilis

Objective To compare the positive rate of difference detection methods on syphilis antibody in serum by TP - ELISA and TPPA methods. Methods Serum samples of 4000 patients were selected. The TP - ELISA and TPPA methods were used to measure positive rate of syphilis antibody. Results The positive rates of syphilis antibody were 3.60% （144/4000） and 3.45% （138/4000） respectively,as detected by TP- ELISA and TPPA methods. Positive coincidence rate was 92.36% （133/144）. There is no difference between two methods when the two method be used for detecting syphilis infection （x2 = 0. 132, P 〉 0.05 ）. Conclusion In clinical detection, TP - ELISA can be used as a screening test for syphilis infection, and TPPA method applies to be further confirmatory test after positive results appearance in order to reduce misdiagnosis and missed diagnosis of syphilis.