| 正交设计法优化黄连总生物碱的提取工艺 |
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| 引用本文： | 张启云,徐国良,孙立波,熊友文,汤喜兰,余日跃,刘红宁.正交设计法优化黄连总生物碱的提取工艺[J].江西中医学院学报,2010,22(3):49-50. |
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| 作者姓名： | 张启云 徐国良 孙立波 熊友文 汤喜兰 余日跃 刘红宁 |
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| 作者单位： | 1. 江西中医学院现代中药制剂教育部重点实验室,南昌,330006
2. 江西中医学院药学院,南昌,330006 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)基金资助项目 |
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| 摘 要： | 目的：考察黄连总生物碱的最佳提取工艺。方法：采用酸性染料比色法测定黄连中总生物碱的含量,通过正交设计方法,以总生物碱的得率为考察指标,优选总生物碱的提取工艺。结果：总生物碱最佳提取工艺为65%乙醇回流提取4次,每次1h,液料比为12∶1。结论：优选得到的工艺使生物碱的提取效率高,稳定性好。
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| 关 键 词： | 黄连 正交设计 总生物碱 |
Optimize the Extraction Technology of Total Alkaloids in Coptis Chinensis by Orthogonal Design |
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| ZHANG Qi-yun,XU Guo-liang,SUN Li-bo,XIONG You-wen,TANG Xi-lan,YU Ri-yue,LIU Hong-ning.Optimize the Extraction Technology of Total Alkaloids in Coptis Chinensis by Orthogonal Design[J].Journal of Jiangxi College of Traditional Chinese Medicine,2010,22(3):49-50. |
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| Authors: | ZHANG Qi-yun XU Guo-liang SUN Li-bo XIONG You-wen TANG Xi-lan YU Ri-yue LIU Hong-ning |
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| Affiliation: | 1.Key Laboratory of Modern Preparation,Ministry of Education,Jiangxi University of Traditional Chinese Medicine,Nangchang330006; 2.School of Pharmacy,Jiangxi University of Traditional Chinese Medicine,Nanchang 330006 |
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| Abstract: | Objective：To identify the best technique of extraction process of total alkaloid from Coptis chinensis. Methods：Acid dye colorimetry was adopted to determine the total alkaloid contents in Coptis chinensis. According to the yield of total alkaoid,the orthogonal design was used to optimize the extraction technology of total alkaloids. Result：The optimum technique was circumfluence extracting four times by 65% alcohol with liquid-solid ratio at 12：1,one hour every time. Conclusion：the optimized technique is efficient and stable. |
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| Keywords: | Coptis chinensis orthogonal design total alkaloids |
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