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Loss of melanopsin-containing retinal ganglion cells in a rat glaucoma model
作者姓名:Wang HZ  Lu QJ  Wang NL  Liu H  Zhang L  Zhan GL
作者单位:WANG Huai-zhou,LU Qing-jun,WANG Ning-li,ZHANG Ling(Tongren Eye Center,Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China);LIU Hong,ZHAN Gui-lin(Department of Ophthalmology & Visual Sciences, University of Nebraska Medical Center, Omaha, NE 68198, USA) 
摘    要:Background Glaucoma can cause progressive damage to retinal ganglion cells. These cells can be classified as cells projecting to the superior colliculus and melanopsin-containing retinal ganglion cells, which project to the suprachiasmatic nucleus. This study was to investigate the effects of chronic intraocular pressure elevation on melanopsin-containing retinal ganglion cells in rats.
Methods Chronic intraocular pressure elevation was induced in one eye of adult Wistar rats by cauterization of three episcleral veins. Intraocular pressure was measured at different intervals with a rebound tonometer. Superior collicular retinal ganglion cells were retrogradely labeled from the superior colliculus with Fluorogold. Melanopsin-containing retinal ganglion cells were visualized by free-floating immunohistochemistry on whole-mount retinas. The number of labeled superior collicular and melanopsin-containing retinal ganglion cells were counted in the sample areas on flat-mounted retinas.
Results Compared with contralateral control eyes, the numbers of both superior collicular and melanopsin-containing retinal ganglion cells were significantly reduced after 12 weeks of experimental intraocular pressure elevation ((2317.41±29.96)/mm^2 vs (1815.82±24.25)/mm^2; (26.20±2.10)/mm^2 vs (20.62±1.52)/mm^2, respectively). The extent of cell loss of the two types of retinal ganglion cells was similar. However, no morphologic changes were found in melanopsin-containing retinal ganglion cells.
Conclusion Both melanopsin-containing and superior collicular retinal ganglion cells were damaged by chronic ocular hypertension, indicating that glaucomatous neural degeneration involves the non-image-forming visual pathway.

关 键 词:青光眼  视网膜神经细胞  眼压  动物模型

China steps up efforts on infectious diseases ahead of Olympics
Wang HZ,Lu QJ,Wang NL,Liu H,Zhang L,Zhan GL.Loss of melanopsin-containing retinal ganglion cells in a rat glaucoma model[J].Chinese Medical Journal,2008,121(11):1024-1024.
Authors:Wang Huai-zhou  Lu Qing-jun  Wang Ning-li  Liu Hong  Zhang Ling  Zhan Gui-lin
Affiliation:1. Tongren Eye Center,Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China
2. Department of Ophthalmology & Visual Sciences, University of Nebraska Medical Center, Omaha, NE 68198, USA
Abstract:BACKGROUND: Glaucoma can cause progressive damage to retinal ganglion cells. These cells can be classified as cells projecting to the superior colliculus and melanopsin-containing retinal ganglion cells, which project to the suprachiasmatic nucleus. This study was to investigate the effects of chronic intraocular pressure elevation on melanopsin-containing retinal ganglion cells in rats. METHODS: Chronic intraocular pressure elevation was induced in one eye of adult Wistar rats by cauterization of three episcleral veins. Intraocular pressure was measured at different intervals with a rebound tonometer. Superior collicular retinal ganglion cells were retrogradely labeled from the superior colliculus with Fluorogold. Melanopsin-containing retinal ganglion cells were visualized by free-floating immunohistochemistry on whole-mount retinas. The number of labeled superior collicular and melanopsin-containing retinal ganglion cells were counted in the sample areas on flat-mounted retinas. RESULTS: Compared with contralateral control eyes, the numbers of both superior collicular and melanopsin-containing retinal ganglion cells were significantly reduced after 12 weeks of experimental intraocular pressure elevation ((2317.41 +/- 29.96)/mm(2) vs (1815.82 +/- 24.25)/mm(2); (26.20 +/- 2.10)/mm(2) vs (20.62 +/- 1.52)/mm(2), respectively). The extent of cell loss of the two types of retinal ganglion cells was similar. However, no morphologic changes were found in melanopsin-containing retinal ganglion cells. CONCLUSION: Both melanopsin-containing and superior collicular retinal ganglion cells were damaged by chronic ocular hypertension, indicating that glaucomatous neural degeneration involves the non-image-forming visual pathway.
Keywords:melanopsin  retinal ganglion cells  intraocular pressure
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