S100A16转基因小鼠的构建与鉴定

目的:构建系统表达S100A16基因型小鼠,为研究S100A16基因的生物学功能提供模型动物?方法:将S100A16 cDNA插入CMV启动子下游,构建转基因表达载体,通过显微注射方法建立转基因小鼠?PCR鉴定转基因小鼠的基因型,采用定量PCR(QPCR)方法筛选高表达品系?结果:成功构建S100A16转基因载体,建立了S100A16转基因小鼠,通过PCR及QPCR方法筛选出两个高表达品系(line A,line B)?结论:建立了系统表达S100A16的转基因小鼠,转入的S100A16基因在脂肪?肝脏?肌肉?肺等组织高表达,为研究S100A16的生物学功能特别是在肥胖中的作用及机制提供了动物模型?

Establishment and identification of S100A16 transgenic mice

Objective:To establish a transgenic mouse model systemic expression S100A16,which can be used for the study of its biologic function. Methods: The plasmid was generated by inserting the murine S100A16 cDNA into a vector with CMV promoter. The transgenic mice were produced by the method of microinjection. The genotype of transgenic lines was identified by PCR and the expression level of the gene was determined by QPCR. Results: S100A16 cDNA transgenic vector was successfully constructed and S100A16 transgenic mice were created. Two high expression lines were determined by QPCR. Conclusion: The transgenic mouse systemic expressing S100A16 was successfully established,and the S100A16 gene was highly expressed in many tissues,such as adipose tissue,liver,muscles,and lung. The S100A16 mouse could be a useful model for the researches of its function,especially in obesity and insulin resistance.