"脾虚证"大鼠下丘脑及结肠组织生长抑素和胆囊收缩素-8含量的变化

目的:观察脾虚证大鼠神经肽含量的变化。方法:大鼠随机分为3组:正常组、模型组和治疗组,每组各10只。采用大黄水煎浓缩剂灌胃建立脾虚证模型,治疗组予四君子汤灌胃治疗;正常组和模型组予生理盐水灌胃。采用放射免疫法测定大鼠下丘脑及结肠组织中生长抑素(somatostatin,SS)和胆囊收缩素-8(cholecystokinin-8,CCK-8)含量;同时测定尿D-木糖(D-xylose)含量。结果:模型组大鼠下丘脑CCK-8含量高于正常组,差异有统计学意义(P<0.05);模型组大鼠结肠黏膜SS含量及CCK-8含量高于正常组,差异均有统计学意义(P<0.05);模型组大鼠结肠黏膜CCK-8含量低于治疗组,差异亦有统计学意义(P<0.05);模型组大鼠尿D-木糖排泄率低于正常组,差异有统计学意义(P<0.05)。结论:脾虚证大鼠神经内分泌调节的紊乱可能是"脾虚-脾失健运"发生发展的重要基础。

Content of somatostatin and cholecystokinin-8 in hypothalamus and colons in a rat model of spleen-deficiency syndrome

Objective:To investigate the changes of neuropeptides content in a rat model of spleen-deficiency syndrome. Methods:Rats were randomly divided into three groups:normal group,untreated group and treatment group. There were ten rats in each group.Spleen-deficiency syndrome was induced by intragastrically administration of rhubarb concentrate in rats of the untreated and treatment group.The rats in the treatment group were intragastrically administered with Sijunzi decoction,a traditional Chinese compound herbal medicine for strengthening spleen and supporting qi.Normal saline was intragastrically administered to the rats in the nor- mal and untreated groups.The somatostatin(SS)and cholecystokinin-8(CCK-8)content in hypothalamus and colons was detected by the method of radioimmunoassay and the D-xylose content in urine was also de- tected. Results:The CCK-8 content in hypothalamus of the untreated group was higher than that of the normal group (P<0.05).SS and CCK-8 content in colons of the untreated group was higher than that of the normal group (P<0.05).The CCK-8 content in colons of the untreated group was lower than that of the treatment group (P<0.05).The excretion rate of D-xylose in the untreated group was lower than that of the normal group (P<0.05). Conclusion:The disorder of neuroendocrine regulation in rats with spleen-deficiency syndrome may be chiefly responsible for"spleen-deficency and dysfunction of spleen"in traditional Chinese medicine.