长链非编码RNA H19对胰腺癌细胞增殖、侵袭及转移的影响

目的: 探讨下调长链非编码RNA H19的表达对胰腺癌SW1990细胞增殖、侵袭转移能力的影响。方法: 从4种胰腺癌细胞Patu8988、SW1990、Bxpc3、Panc-1中筛选出H19高表达株与低表达株。对高表达H19的SW1990细胞株瞬时转染H19 siRNA(实验组)，同时转染阴性siRNA作为对照组。采用qRT-PCR检测转染效率；CCK-8法检测细胞增殖活性；平板克隆法检测细胞克隆形成能力；Transwell试验检测细胞迁移和侵袭的变化；蛋白质印迹法检测细胞增殖和凋亡相关蛋白的表达。结果： 与对照组相比，实验组H19 mRNA表达明显降低(P<0.05)，细胞相对增殖率无变化(P>0.05)，但细胞克隆形成数、细胞侵袭及转移能力明显提高，ZEB1 mRNA表达增加，但mir-200家族的表达无明显变化(P>0.05)，ZEB1、Snail及PCNA蛋白表达增加，Bcl/Bax比值升高(P均<0.05)。结论： 下调H19表达可提高细胞克隆形成能力，促进细胞侵袭转移。

Effects of long non-coding RNA H19 expression on cell proliferation,migration and invasion of pancreatic cancer cells

Objective To investigate the effects of long-coding RNA H19 on proliferation, migration and invasion of pancreatic cancer cells. Methods We first detected the expression of H19 in Patu8988, SW1990, Bxpc3 and Panc-1 pancreatic cancer cells with real-time quantitative PCR(qRT-PCR). The SW1990 and Bxpc3 cells had higher H19 expression while Patu8988 and Panc-1 had lower H19 expression. The H19 siRNAs were transfected into pancreatic cancer cells SW1990 in vitro. The mRNA expression of ZEB1 and mir-200 were analyzed with qRT-PCR. The effects of H19 on proliferation were evaluated by CCK-8 and colon formation assays. The expression of cell proliferation- and EMT-related proteins were detected with Western blotting. Results Compared with the control group, SW1990 cells transfected with H19 siRNAs revealed a significant lower level of H19 and lower the ability of cell colony formation. However, there was no significant change on cell proliferation rate; the expressional levels of ZEB1,Snail and PCNA were up-regulated as well as the ratio of Bcl-2 to Bax; the mRNA level of ZEB1 was up regulated while the expression of miR-200 showed no significant change. Conclusion Down regulation of H19 promotes cell proliferation, migration and invasion in pancreatic cancer cells SW1990. ［Key words］H19; ZEB1; mir-200; cell migration; cell invasion