| 乙型肝炎病毒转基因小鼠中外源基因整合的检测 |
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| 引用本文： | 贾彦征,刘光泽,熊一力,王洪敏,王添章,张宜俊,邵启祥.乙型肝炎病毒转基因小鼠中外源基因整合的检测[J].江苏大学学报(医学版),2000(2). |
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| 作者姓名： | 贾彦征 刘光泽 熊一力 王洪敏 王添章 张宜俊 邵启祥 |
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| 摘 要： | 目的 :了解乙型肝炎病毒DNA序列在乙型肝炎病毒转基因小鼠中的整合情况。方法 :用头尾相接的乙型肝炎病毒全序列基因 ,通过原核显微注射法产生转基因小鼠 ,用C区特异性引物扩增鼠尾DNA检测整合 ,对其中 10只阳性鼠的扩增产物测序。结果 :12 8只仔鼠中 ,14只整合阳性。对 10只阳性鼠测序 ,9只与所转基因序列完全相同 ,1只在173 9、1740位缺失两个碱基。结论 :外源乙型肝炎病毒基因确实整合至小鼠基因组 ,多数为正常整合 ,但也存在缺失整合。
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| 关 键 词： | 转基因小鼠 乙型肝炎病毒 整合 |
Detection of the Integration of Exogenous DNA in Hepatitis B Virus Transgenic Mice |
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| JIA Yan zheng,LIU Guang ze,XIONG Yi li,et al.Detection of the Integration of Exogenous DNA in Hepatitis B Virus Transgenic Mice[J].Journal of Jiangsu University Medicine Edition,2000(2). |
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| Authors: | JIA Yan zheng LIU Guang ze XIONG Yi li |
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| Abstract: | Objective:To investigate the integration of exogenous DNA in hepatitis B Virus (HBV) transgenic mice. Methods:Transgenic mice carrying HBV gene were generated by means of pronucleus injection. Using HBV Core gene primer to amplify tail tissue DNA, the PCR pruducts of 10 positive transgenic mice were sequenced. Results:14 out of 128 offsprings were HBV positive,9 out of the 10 positive mice had the same sequence as the injected HBV DNA,while one transgenic mice had 2 nucleotide (nt) deletions in 1739nt and 1740nt.Conclusions:It was showed that exogenous HBV DNA had integration into mice genome. |
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| Keywords: | Transgenic mice HBV Integration |
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