| 雌激素抑制大鼠肝癌肺转移机制研究 |
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| 引用本文： | 王永仓,许戈良,赵旭辉,魏忠,樊平,戴寅,谢钊,刘巧玉.雌激素抑制大鼠肝癌肺转移机制研究[J].安徽医学,2015,36(12):1439-1441. |
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| 作者姓名： | 王永仓 许戈良 赵旭辉 魏忠 樊平 戴寅 谢钊 刘巧玉 |
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| 作者单位： | 230031,合肥 安徽省肿瘤医院腹部肿瘤外科;230001,合肥 安徽省立医院肝脏外科 |
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| 摘 要： | 目的探讨雌激素对大鼠肝癌肺转移形成的影响及其可能的机制。方法将雌性大鼠随机分为切除卵巢组、对照组、假手术组和去卵巢后加雌激素组，建立大鼠肝癌模型。诱癌20周后观察肿瘤发生、肺转移的情况；免疫组织化学法检测4组成瘤大鼠肝癌组织中雌激素受体α（ERα）、磷酸化雌激素受体α（P-ERα）、肝细胞生长因子（HGF）和白介素6（IL-6）的表达；半定量逆转录聚合酶链反应法检测 ERα、HGF 及 IL-6的 mRNA 水平。结果切除卵巢组大鼠肝癌肺转移发生率明显高于其他3组（P ＜0.05）；大鼠肝癌肺转移发生率越高，肝癌组织中 HGF、IL-6的蛋白及分子水平表达越高（P ＜0.05），而 ERα的蛋白及分子水平表达越低（P ＜0.05）。结论雌激素可能通过下调 HGF 和 IL-6的表达来抑制肝癌肺转移的发生。
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| 关 键 词： | 肝细胞肝癌 肝癌肺转移 雌激素 炎症因子 |
| 收稿时间： | 2015/5/19 0:00:00 |
| 修稿时间： | 2015/7/3 0:00:00 |
The mechanism study of inhibition of hepatic tumor lung metastasis by estrogen in a rat model |
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| Wang Yongcang,Xu Geliang,Zhao Xuhui.The mechanism study of inhibition of hepatic tumor lung metastasis by estrogen in a rat model[J].Anhui Medical Journal,2015,36(12):1439-1441. |
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| Authors: | Wang Yongcang Xu Geliang Zhao Xuhui |
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| Affiliation: | Department of Abdominal Tumor Surgery, Anhui Provincial Tumor Hospital, Hefei 230031, China,Department of Abdominal Tumor Surgery, Anhui Provincial Tumor Hospital, Hefei 230031, China,Department of Abdominal Tumor Surgery, Anhui Provincial Tumor Hospital, Hefei 230031, China,Department of Abdominal Tumor Surgery, Anhui Provincial Tumor Hospital, Hefei 230031, China,Department of Abdominal Tumor Surgery, Anhui Provincial Tumor Hospital, Hefei 230031, China,Department of Abdominal Tumor Surgery, Anhui Provincial Tumor Hospital, Hefei 230031, China and Department of Abdominal Tumor Surgery, Anhui Provincial Tumor Hospital, Hefei 230031, China |
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| Abstract: | Objective To determine whether and how estrogen reduced lung metastasis of HCC in a rat model. Methods We con-ducted a rat HCC model in female rats induced by diethylnitrosamine(DEN)and N-nitrosomorpholine(NMOR),and rats were distributed into four groups:ovariectomy,sham operation,ovariectomy followed by 30 μg/(kg·d)body weight/ day 17α-Ethynylestradiol(EE2)sup-plementation,sexually intact controls. Results Here we showed that,60% lung metastasis was observed in the rats of ovariectomy group, whereas obviously lower lung metastasis was found in the rats of other three groups. Furthermore,the higher expression of interleukin-6(IL-6), hepatocyte growth factor(HGF)and the lower expression of estrogen receptor α(ERα),the higher lung metastasis of HCC we founded. Con-clusion Estrogen may inhibit hepatic tumor lung metastasis by suppression of HGF and IL-6 production. |
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| Keywords: | Hepatocellular carcinoma Lung metastasis Estrogen Inflammatory factor |
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