益肾降浊冲剂对慢性肾功能衰竭大鼠肾小管上皮细胞转分化的影响

目的观察益肾降浊冲剂对慢性肾衰(CRF)大鼠肾小管上皮细胞向间充质细胞转分化(EMT)的影响,探讨其延缓CRF进展的作用机制。方法采用单侧肾切除加阿霉素重复注射建立慢性肾衰大鼠模型,随机分为假手术组、模型组、缬沙坦、益肾降浊冲剂治疗组。治疗组分别予缬沙坦、益肾降浊冲剂灌胃8周,Masson染色观察肾小管间质胶原沉积的程度,免疫组化检测肾小管上皮细胞TGF-β1、α-SMA的表达。结果模型组残肾肾小管出现萎缩、扩张、胶原染色阳性面积明显高于假手术组,TGF-β1、α-SMA的表达明显上调(P<0.01);缬沙坦组、益肾降浊冲剂组胶原染色阳性面积及TGF-β1、α-SMA的表达量均明显低于模型组(P<0.01);益肾降浊冲剂组胶原染色阳性面积减少及TGF-β1、α-SMA的表达量均明显低于缬沙坦组(P<0.05)。结论益肾降浊冲剂能通过抑制CRF残肾小管上皮细胞TGF-β1的表达、进而抑制EMT及胶原的沉积,作用优于缬沙坦。

Effects of Yishen Jiangzhuo Granule on Renal Tubular Epithelial Cells Transdifferentiation in Chronic Renal Failure Rats

Objective To observe the effect of Yishen Jiangzhuo granule on epithelial-mesenchymal transition(EMT) of renal tubular cells in chronic renal failure(CRF) rats,and explore its mechanism of delaying the advance of CRF.Methods CRF rat models were induced by uninephrectomy and duplicate injection of adriamycin.The rats were randomly divided into sham operation group,model group,valsartan group and Yishen Jiangzhuo granule group,and the later two groups were given respectively valsartan,Yishen Jiangzhuo granule by gavage.After 8 weeks,the degree of collagen deposition in renal tubular interstitium was observed by Masson staining,and the expressions of TGF-β1 and α-SMA in renal tubular epithelial cells were detected by immunohistochemisty.Results The kidney tubules of remnant kidney appeared to atrophy and extension in model group,and the area of positive collagen staining and the expressions of TGF-β1 and α-SMA were significantly higher than that in the control group(P<0.01).The area of positive collagen staining and the expressions of TGF-β1 and α-SMA in valsartan group,Yishen Jiangzhuo granule group were significantly lower than that in the model group respectively(P<0.01).The area of positive collagen staining and the expressions of TGF-β1 and α-SMA in Yishen Jiangzhuo granule group were significantly lower than that in the valsartan group respectively(P<0.05).Conclusion Yishen Jiangzhuo granule could inhibit EMT and collagen deposition by inhibiting the expression of TGF-β1 in renal tubular epithelial cells of CRF rats remnant kidney,and the effect is better than valsartan.