人巨细胞病毒在人胚肺成纤维细胞内的形态发生的观察

人巨细胞病毒接种人胚肺成纤维细胞后20分钟,可见病毒吸附和穿透过程。感染后18小时,在核内已出现包涵体,在其网眼内和网架上有核衣壳复制和装配。胞浆包涵体是病毒成熟时在胞浆中引起的一种细胞局灶性变性。溶酶体只吞噬和灭活小部分病毒,大部分病毒和致密体在囊泡内成熟时已获得包膜。它们以胞吐方式和细胞溶解方式释放到细胞外。

Primary Observations of the Morphogenesis of Cytomegalovirus in Human Cell Culture

Absorption and penetration of virus were detected within 20 min. after inoculation by strain of HCMV AD 169. At the 18th hr. after infection the development of nuclear inclusion in human embryonic lung fibroblast was noted. The replication of capsid and percusor of viral core were observed in the network of nuclear inclusion here and there. Cytoplasmic inclusion actually is an intracellular local degenelation of infected cells by HCMV, which is associated with the maturation of virus. Owing to active phagocytosis of lysosome, the small part of enveloped capsids and dense bodies were degraded and inactivated by lysosomal enzymes, the major part of mature viral particles and dense bodies in vaculoe did not need to acquire an envelop by budding directly into lysosome.