幽门螺杆菌VacA基因型与胃疾病

目的:探讨幽门螺杆菌(H.pylori)VacA基因型在各种胃肠疾病中的分布及关系.方法:从胃十二指肠疾病患者胃粘膜标本中分离培养H.pylori,PCR测定VacA基因型.结果:108株H.pylori菌株,VacA s1a阳性为85株(79%),VacA s1b阳性22株(20%),VacA m1阳性29株(27%),VacA m2阳性77株(71%),VacA s1a/m2阳性66株(61.1%),未发现s2型菌株.其中,慢性胃炎患者VacA s1a阳性率为65.4%(34/52),消化性溃疡患者VacA s1a阳性率为90%(36/40),胃癌患者VacA s1a阳性率为94%(15/16).结论:H.pylori菌株VacA基因型绝大多数为s1a/m2型,该型菌株存在于各种胃肠疾病中,无s2型.

Relationship between VacA genotypes of Helicobacter pylori and gastric diseases

Objective:To investigate the distribution of Helicobacter pylori (H.pylori) VacA genotypes in different gastrointestinal diseases and the relationship among each other.Methods:VacA genotypes of 108 H.pylori strains isolated from patients with chronic gastritis,peptic ulcer and gastric cancer were tested by polymerase chain reaction (PCR).Results:The frequencies of the signal allele sla,slb and the middle-region allele m1,m2 were 85 (79%),22 (20%),29 (27%),77 (71%) respectively.The numbers of H.pylori strains of VacA s1a/m2 positive were 66 (61.1%).The s2 form was not found.The positive rates of VacA s la of H.pylori strains isolated from patients with chronic gastritis,peptic ulcer and gastric cancer were 65.4% (34/52),90% (36/40) and 94% (15/16) respectively.Conclusion:The majority of H.pylori strains isolated from Chongqing patients express s1a/m2.The s1a/m2 genotype is dispersed in all of the gastrointestinal diseases associated with H.pylori.However the s2 form is not found.