| 5-氟尿嘧啶联合 DC-CIK 细胞对胃癌细胞杀伤作用的体外研究简 |
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| 引用本文： | 张娜,杜昱蕾,李晶,胡增祥,王新胜,李薇.5-氟尿嘧啶联合 DC-CIK 细胞对胃癌细胞杀伤作用的体外研究简[J].白求恩军医学院学报,2014,0(1):3-5. |
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| 作者姓名： | 张娜 杜昱蕾 李晶 胡增祥 王新胜 李薇 |
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| 作者单位： | 张娜 (河北省承德医学院研究生处, 承德,067000)； 杜昱蕾(解放军260 医院肿瘤内分泌科, 石家庄,050041)； 李晶 (河北省承德医学院研究生处, 承德,067000)； 胡增祥(解放军260 医院肿瘤内分泌科, 石家庄,050041)；王新胜(解放军260 医院肿瘤内分泌科, 石家庄,050041)；李薇(解放军260 医院肿瘤内分泌科, 石家庄,050041)； |
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| 基金项目： | 国家自然科学基金资助项目(项目编号：编号30800514) |
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| 摘 要： | 目的：观察5-氟尿嘧啶（5-FU）联合DC-CIK细胞对胃癌BGC-823细胞及其侧群（side population，SP）细胞的体外杀伤效应。方法从胃癌BGC-823细胞中分离获得BGC-823-SP细胞，将BGC-823细胞设为A组，BGC-823-SP细胞设为B组；取健康人外周血单核细胞，培养DC-CIK细胞；用MTT比色法测定5-FU、DC-CIK细胞对A、B 两组胃癌细胞的杀伤率，并测定两者联合对A、B两组胃癌细胞的杀伤率。结果①不同浓度5-FU对A组胃癌细胞杀伤率均高于B组，即B组比A组对5-FU的耐药性更强；②不同效靶比DC-CIK细胞对两组细胞均有较强杀伤作用，且随着效靶比的增加杀伤效果逐渐增强。同一效靶比下，DC-CIK细胞对A组与B组胃癌细胞的杀伤率比较，差异均无统计学意义（ P ＞0．05）；③5-FU联合DC-CIK细胞对两组胃癌细胞的杀伤率均明显高于单独应用5-FU或DC-CIK细胞。结论5-FU联合DC-CIK细胞能够显著抑制胃癌BGC-823细胞及其BGC-823-SP细胞的生长，可为临床化疗联合生物免疫治疗胃癌提供理论依据。
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| 关 键 词： | 胃癌 侧群细胞 DC-CIK细胞 5-氟尿嘧啶 |
The anti-tumor effect of 5-Fluorouracil combined with DC-CIK cells against gastric carcinoma cells in vitro |
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| Affiliation: | ZHANG Na, DU Yulei, LI Jing, et al. Department of Postgraduates, Chengde Medical College, Hebei 067000, China |
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| Abstract: | Objective To observe the inhibitory effect of 5-Fluorouracil（5-FU）combined with DC-CIK cells against gastric carcinoma cell line BGC-823 and the side population（SP） cells in vitro.Methods BGC-823-SP cells were isolated from gastric carci-noma cell line BGC-823 .And BGC-823 cells were set as group A and BGC-823-SP cells as group B .DC-CIK cells were generated by stimulating human peripheral blood mononuclear cells .Then MTT colorimetric assay was used to evaluate the killing rates of 5-FU,DC-CIK cells and their combination against the gastric carcinoma cells of group A and B .Results ①The killing rates of different concen-trations of 5-FU in group A were both higher than those in group B , i.e.the drug resistance to 5-FU of group B was stronger than that in group A.②DC-CIK cells could inhibit both BGC-823 cells and BGC-823-SP cells,and the inhibitory effects were gradually en-hanced with the increase of effect target ratio .At the same effect target ratio ,there were no statistical differences in DC-CIK cells of group A and group B （ P ＆gt;0.05）.③The killing rates of 5-FU combined with DC-CIK cells against the two groups gastric carcinoma cells were both higher than that of single treatment group .Conclusion 5-FU combined with DC-CIK cells can significantly inhibit the growth of gastric carcinoma cells and the SP cells ,and the results provide the theoretical basis for the clinical application of chemothera -py combined with immunotherapy in inhibition of gastric carcinoma . |
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| Keywords: | Gastric carcinoma Side population cells DC-CIK cell 5-Fluorouracil |
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