黄花菜根多糖的超声提取及含量测定

目的探讨超声波法提取黄花菜根多糖最佳条件,并测定其含量。方法通过单因素实验和正交试验,确定超声提取最佳工艺。用苯酚-硫酸法测定黄花菜根多糖的含量。结果正交试验结果表明各因素影响程度依次为：提取温度〉超声功率〉超声时间〉料液比,超声波法提取黄花菜根多糖最佳条件为：超声时间10 min,超声功率70 Hz,料液比1∶20,浸提温度70℃。在此参数条件下多糖的提取率达15.95%。结论超声波法提取黄花菜根多糖具有省时、节能、提取率高等优点。

The extraction and content determination of polysaccharide of the root of the Hemerocallis fulva by the supersonic method

Objective To study the technology of extraction of polysaccharide from the root of the Hemerocallis fulva and determine its contents.Methods Single factor and orthogonal experiment were applied to analyze the effects of the factors such as temperature,supersonic power,time and solid-liquid ratio on the extraction percent of the polysaccharides.Results Experimental results indicated that temperature and supersonic power significantly affect the extraction percent of the polysaccharides.The optimum ultrasonic...