HBV S基因与IL-12基因联合免疫小鼠的免疫应答

目的 观察小鼠对IL-12基因表达质粒与HBVS基因疫苗联合免疫的免疫应答。方法 用已构建的HBVS基因疫苗（pCR3.1-S)和表达IL-12p35和p40蛋白的真核表达载体pWRG3169分别给BALB/c小鼠多点肌肉注射，2wk后追加免疫1次，用ELISA法及MTT法检测小鼠血清抗-HBs及脾细胞对HBsAg的特异性增殖反应。结果 免疫接种2wk后小鼠血甭抗-HBs滴度及脾细胞对HBsAg的刺激指数均明显高于对照组，pWRG3169 pCR3.1-S组明显高于单纯pCR3.1-S注射组。结论 IL-12表达质粒可以增强pCR3.1-S基因疫苗诱导的体液和细胞免疫应答强度，尤以细胞免疫增高明显。

Enhanced immune responses in BALB/c mice coimmunized with IL-12 gene expression plasmid and hepatitis B virus surface DNA-based vaccine

AIM To study enhanced immune response in mice coimmunized with HBV surface DNA based vaccine and IL 12 gene expression plasmid. METHODS The HBV surface gene was cloned into a eukaryotic expression vector (pCR3.1 S). The Balb/c mice were immunized at multiple sites by intramuscular injection with pCR3.1 S and IL 12 gene expression plasmid (pWRG3169). Each mouse was boosted 2 weeks after the immunization. The anti HBs antibody was detected by ELISA and the stimulatory index of splenocytes on HBsAg was measured by MTT method. RESULTS The titer of anti HBs antibody and the stimulatory index of splenocytes from mice coimmunized with pWRG3169 and PCR3.1 S was significantly higher than that of the mice injected with pCR3.1 S alone. Antibody titer and SI of immunized mice were higher than those of the control group. CONCLUSION This study demonstrates that coimmunization of IL 12 gene expression plasmid and HBV surface gene can enhance humoral and cellular immune response of immunized mice.