| 金属硫蛋白基因对小鼠变应性接触性皮炎的影响 |
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| 引用本文： | 路雪艳,李林峰,王文慧,张宝旭.金属硫蛋白基因对小鼠变应性接触性皮炎的影响[J].北京大学学报(医学版),2003,35(2):184-186. |
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| 作者姓名： | 路雪艳 李林峰 王文慧 张宝旭 |
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| 作者单位： | 1. 北京大学第三医院皮肤科,北京,100083
2. 北京大学公共卫生学院毒理学系,北京,100083 |
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| 基金项目： | 面向21世纪教育振兴行动计划(985计划);; |
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| 摘 要： | 目的：探讨金属硫蛋白（metallothioneins,MTs)在小鼠变应性接触性皮炎中所起的作用。方法：用二硝基氟苯（dinitrofluorobenzene,JNFB)致敏金属硫蛋白基因敲除小鼠（metallothionein-null mouse,MT-/-,不表达MT－I及MT－Ⅱ）及基因同源对照野生型鼠（MT＋／＋，正常表达MTs)，并在小鼠右耳激发变应性接触性皮炎，分别对比两组小鼠激发后耳肿胀度。结果：野生型鼠（MT＋／＋）耳肿胀度明显高于基因敲除鼠（MT－／－），统计学分析差异有显著性（P＜0．05，t检验）。结论：MTs在迟发型变态反应的炎症过程中起重要作用，MT－／－小鼠不能完全表达迟发型变态反应的炎症。
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| 关 键 词： | 金属硫蛋白 皮炎 变应性接触性 基因敲除小鼠 |
| 文章编号： | 1671-167X(2003)02-0184-03 |
Metallothioneins gene in induced mouse allergic contact dermatitis |
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| Xueyan Lu,Linfeng Li,Wenhui Wang,Baoxu Zhang.Metallothioneins gene in induced mouse allergic contact dermatitis[J].Journal of Peking University:Health Sciences,2003,35(2):184-186. |
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| Authors: | Xueyan Lu Linfeng Li Wenhui Wang Baoxu Zhang |
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| Affiliation: | Department of Dermatology, Peking University Third Hospital, China. |
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| Abstract: | OBJECTIVE: To investigate the chronic hypersensitivity (CHS) to dinitrofluorobenzene (DNFB) in metallothionein gene knocked-out (MT -/-) mice and wild mice (MT +/+) with homologous genes. METHODS: Mice were sensitized by applying DNFB on abdominal skin and CHS was induced on right ears. The CHS was observed by ear swelling. RESULTS: In comparison with metallothionein--null mice, the ear swelling of normal control wild mice was significantly greater. (P < 0.05, Students t test). CONCLUSION: Metallothioneins play an important role in the CHS. Metallothionein gene knocked-out (MT -/-) mice express less inflammation of CHS. |
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| Keywords: | Metallothioneins Dermatitis allergic contact Gene knocked out mouse |
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