| A New System for Rapid Measurement of ATP |
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| 作者姓名： | 舒柏华 周宜开 任恕 |
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| 作者单位： | Institute of Environmental Medicine，Tongji Medical University，Wuhan 430030 |
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| 摘 要： | TheATPthatplaysanimportantrole'inbiologicalactivityofcells,whichisnotnonlytheenergysourceoflifebutalsoakindofregulatingagentofmanybioactivesubstances.So,itisofgreatimportancetomeasureATPrapidlyandpreciselyinthefieldsofbasicmedicine,clinicalmedicine,andpreventivemedicine.Presently,thebestmethodformeasuringATPisblolumlnescentanalysis'].Themethodistomeasureluminousintensityafterinjectionluciferase.Itneedsavastamountofexpensiveluciferase2'3.4J.Theinstrumentiscomplex,oflargesizeandexpensive.…
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| 收稿时间： | 4 September 1996 |
A new system for rapid measurement of ATP |
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| Shu Baihua,Zhou Yikai,Ren Shu.A New System for Rapid Measurement of ATP[J].Journal of Zuazhong University of Science and Technology: Medical Edition,1997,17(3):190-192. |
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| Authors: | Shu Baihua Zhou Yikai Ren Shu |
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| Affiliation: | (1) Institute of Environmental Medicine, Tongji Medical University, 430030 Wuhan |
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| Abstract: | The paper introduces a new type instrument for rapid measuring ATP.The system consists of a micromodule ATP sensor and an instrument for measuring weak light transmitted by optic fiber. The micromodule ATP sensor mainly is composed of enzyme membrane, a probe and a bundle of optic fiber. The instrument measuring weak light consists of photomultiplier, high voltage power, pulse amplifier and counter. The instrument was characterized by simple structure,small size, rapid response time (<5 s), high sensitivity (10-12 mol/L), stable performance (measuring the same sample for 50 times, CV< 5% ), long enzyme storage time (>3 months). |
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| Keywords: | adenosine triphosphate luminescent analysis instrument sensor |
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