己酮可可碱治疗非乙醇性脂肪性肝炎的实验研究

目的探讨己酮可可碱(PTX)对非乙醇性脂肪性肝炎(NASH)的治疗作用。方法将实验性脂肪性肝炎模型大鼠,随机分为正常对照组、模型组、治疗组3组。模型组、治疗组大鼠继续高脂饲养,治疗组予以PTX16mg/(kg·d)治疗4周。对照组大鼠普通饲料喂养。实验16周时处死3组大鼠,分别检测体质量、肝指数、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、三酰甘油(TG)、胆固醇(TC),并进行HE、油红O和Masson染色观察肝脏病理改变,进行炎症活动度计分。结果模型组大鼠体质量、肝指数明显高于正常对照组(P<0.01),PTX治疗后体质量及肝指数均显著降低(P<0.01);模型组大鼠血清ALT、AST与正常对照组比较差异有统计学意义(P<0.01),治疗组大鼠血清AST与模型组比较差异有统计学意义(P<0·01)。模型组TC明显高于对照组(P<0·05),治疗组TG、TC均降低,但差异无统计学意义。组织学观察显示治疗组肝组织炎症坏死程度减轻,炎症活动度计分明显下降(P<0·01)。结论PTX可改善高脂饮食诱发的脂肪性肝炎大鼠肝脏酶学及组织损伤,对实验性NASH具有治疗作用。

The Experimental Study of Nonalcoholic Steatohepatitis Treated with Pentoxifylline

Objective The aim of this study was to investigate the efficacy of pentoxifylline in the treatment of NASH induced by high-fat diet.Methods A nonalcoholic steatohepatitis model was prepared by feeding rats with fat-rich diet for 12 weeks, then these rats were sacrificed. NASH was confirmed by liver histologic exam, then the rats were divided into 3 groups randomly, i.e. normal control group, model group and treatment group. The model group and treatment group were given high-fat diet and the normal group was given normal diet continuously. Furthermore,the rats of treatment group were given pentoxifylline16 mg/(kg·d) for 4 weeks. At the time of 16 weeks, the rats of three groups were sacrificed. The body weight, liver index(liver/body weight ratio), serum ALT,AST,TG,TC were measured and the liver histology of rats of all groups were examined with HE, Masson and oil red stain, and hepatic inflammation score was estimated.Results The body weight and liver index of model group were greatly higher than normal groups (P<0.01), but the body weigh and liver index of treatment group were increasingly dropped (P<0.01). The difference of the serum levels of ALT and AST between model group and normal group's was significant (P<0.01). Compared with normal group, The serum levels of AST in treatment group have significant difference (P<0.05). The serum level of TC of model group was increased significantly (P<0.05), the serum levels of TC,TG of treatment group were dropped, but there was no statistical significance and liver histology was showed hepatic inflammation score descended markedly (P<0.01).Conclusion Pentoxifylline could ameliorate the hepatic inflammation of rats with steatohepatitis induced by fat-rich diet and it suggests that pentoxifylline might be effective in treating rats with nonalcoholic steatohepatitis induced by high fat feeding.