旋毛虫成虫部分cDNA表达质粒文库的构建

目的为寻找更多有效的旋毛虫病疫苗候选抗原分子,构建旋毛虫成虫部分cDNA质粒文库。方法以旋毛虫成虫cDNA文库为模板,进行PCR扩增。扩增产物500~2 000 bp之间的DNA片段用BamHⅠ、HindⅢ酶切,将酶切产物克隆入真核表达载体pcDNA3.1,对部分转化子进行酶切鉴定。结果构建了插入片段在500~1 500 bp之间的旋毛虫成虫部分cDNA质粒文库,90%以上的转化子都含有插入的DNA片段。结论成功构建一个旋毛虫成虫部分cDNA质粒文库。

Construction of Partial Adult cDNA Plasmid Library from Trichinella spiralis

Objective To find out more effective vaccine candidate antigen against the Trichinella spiralis,and to construct the adult cDNA plasmid library of T.spiralis.Methods The cDNA fragments were amplified using adult cDNA plasmid library as the template by PCR and then the fragments digested with BamHⅠand HindⅢ to the size of 500～1500 bp were cloned into a eukaryotic expression vector pcDNA3.1.The inserted DNA fragments in the recombinants were identified by digestion with restriction enzyme.Results The partial adult cDNA plasmid library of T.spiralis,with 10⁵ transformants,was constructed.Most of the recombinants contained the insert DNA fragments.Conclusion A partial adult cDNA plasmid library of T.spiralis is constructed successfully.