低氧对胆管癌细胞增殖的影响及与HIF-1α表达的关系

目的 建立缺氧模型,观察缺氧对QBC939胆管癌细胞生长和HIF-1 α表达的影响.方法 利用氯化钴(cobalt chloride,CoCl2)模拟缺氧诱导,MTT观察缺氧诱导对胆管癌细胞增殖的影响,用流式细胞术观察缺氧对细胞周期的影响,利用Western Blot观察缺氧诱导对胆管癌细胞HIF-1 α表达的影响.结果 CoCl2(0μmol/L,50μmol/L,100μ mol/L,200μ mol/L,400μmol/L)浓度依赖性诱导胆管癌细胞增殖；CoCl2(200μmol/L)处理48 h组QBC939胆管癌细胞G1期显著减少,处理72 h组G1期显著减少的同时S期和G2显著增加；CoCl2(50μmol/L,100μmol/L,200μmol/L)处理72 h,HIF-1 α表达显著增加,并随CoCl2浓度增加显著增强.结论 缺氧可诱导胆管癌细胞增殖与增加HIF-1α表达相关.

The proliferation effect of QBC939 cell induced by hypoxia and the expression of HIF-1α

【Objective】 To establish the hypoxia model and observe the reflectance of QBC939 cell proliferation and HIF-1α expressions induced by hypoxia.【Methods】 The hypoxia model was induced by the usage of cobalt chloride(CoCl2) and MTT test was used to observe the hypoxia influence of QBC939 cell growth curve.Cell cycle affected by CoCl2 was assessed by flow cytometry.Western Blot and immunofluorescence staining were performed to determine the HIF-1α expression of hypoxia QBC939 cell.【Results】 Hypoxia can stimulate the QBC939 cell proliferation in a certain cobalt chloride concentration(<400 μmol/L).The percentage of cells in G1 phase began to decrease after a 48 h CoCl2 treatment at 200μmol/L,which in S and G2 phase increased significantly after a 72h treatment.The HIF-1α expression of QBC939 cell had significant correlation with the CoCl2 concentration(P <0.05).【Conclusion】 Hypoxia can effectively increase the proliferation of QBC939 cells.The underlying mechanism may be due to the up-regulation of HIF-1α,which lead to a cell cycle arrest in G0/G1 phases,and finally inhibit the proliferation.