| 反向克隆法大鼠anti- ERK2腺病毒载体构建 |
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| 引用本文： | 董冲,刘云,宫念樵,陈曦林,唐莉,朱国超,陈知水,叶启发.反向克隆法大鼠anti- ERK2腺病毒载体构建[J].中国现代医学杂志,2006,16(1):21-23. |
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| 作者姓名： | 董冲 刘云 宫念樵 陈曦林 唐莉 朱国超 陈知水 叶启发 |
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| 作者单位： | 1. 华中科技大学同济医学院附属同济医院器官移植研究院教育部/卫生部重点实验室,湖北,武汉,430030
2. 中南大学湘雅三医院器官移植研究院,湖南,长沙,410008 |
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| 摘 要： | 目的正义基因反向克隆法构建大鼠anti-ERK2腺病毒载体.方法酶切质粒p3XFLAG-CMV7.1-ERK2,得到ERK2 cDNA片段,连接到T载体进行测序,经测序正确反向克隆法插入质粒pShuttle中,最后转入AdEasy(tm)XL Adenoviral Vector System系统中得到anti-ERK2基因腺病毒载体.结果DraI和NotI双酶切鉴定anti-ERK2基因腺病毒载体,发现插入的基因序列与插入方向均符合预期目标.结论成功构建了大鼠anti-ERK2腺病毒载体,为研究移植排斥中ERK2基因治疗的作用提供了良好工具.
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| 关 键 词： | 反向克隆 腺病毒载体 |
| 文章编号： | 1005-8982（2006）01-0021-03 |
| 收稿时间： | 2005-10-09 |
| 修稿时间： | 2005-10-09 |
Construction of recombinant adenoviral vector carrying rat anti-ERK2 gene by reverse orientation clone technology |
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| DONG Chong,LIU Yun,GONG Nian-qiao,CHEN Xi-lin,TANG Li,ZHU Guo-chao,CHEN Zhi-shui,YE Qi-fa.Construction of recombinant adenoviral vector carrying rat anti-ERK2 gene by reverse orientation clone technology[J].China Journal of Modern Medicine,2006,16(1):21-23. |
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| Authors: | DONG Chong LIU Yun GONG Nian-qiao CHEN Xi-lin TANG Li ZHU Guo-chao CHEN Zhi-shui YE Qi-fa |
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| Affiliation: | 1.Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R.China; 2. Institute of Organ Transplatation, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R.China |
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| Abstract: | |
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| Keywords: | anti-ERK2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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