肝细胞癌患者血浆TF,uPA及uPAR检测及相关分析

目的 探讨肝细胞癌患者血浆TF,uPA和uPAR水平变化及临床病理意义.方法 应用酶联免疫法(ELISA)检测肝细胞癌患者50例及对照组30例的血浆TF,uPA和uPAR水平并结合临床病理资料探讨其统计学意义.结果 肝细胞癌患者血浆TF,uPA及uPAR均较对照组升高,差异有显著性(P＜0.05).肝细胞癌患者血浆TF在低分化组,肿瘤较大组及合并肝硬化组显著升高(P＜0.05),而在不同癌灶数目及包膜情况组间差异无显著性(P＞0.05).血浆uPA水平只在合并肝硬化组升高(P＜0.05),uPAR水平与以上病理指标均无关(P＞0.05).肝细胞癌患者血浆TF,uPA和uPAR水平均与侵袭转移指标有关,在有淋巴转移,肝外脏器转移及门脉癌栓组较无转移及癌栓组升高(P＜0.05).结论 肝细胞癌患者存在凝血,纤溶激活状态.血浆TF,uPA和uPAR升高与肝细胞癌的发生及侵袭转移有关,其检测有助于肝癌早期诊断及预后判断.

Detection and analyses of TF, uPA and uPAR levels in plasma of hepatocellular carcinoma patients

Objective] To study the levels change of plasma TF, uPA and uPAR levels and elucidate their association with clinical significance clinical significance in Hepatocellular Carcinoma (HCC). Methods] Blood samples were obtained from 50 patients of HCC and 30 cases of control. Plasma levels of TF, uPA and uPAR of all samples were detected by ELISA. The relationships between the three factors and clinic-pathological data were analyzed by SPSS software. Results] Plasma TF, uPA and uPAR levels were all increased significantly in HCC group when compared with control (P <0.05). TF levels were higher in poor differentiation, large size and cirrhosis subgroup in HCC patients (P <0.05) but similar in different tumor number or envelope. uPA levels were not significantly different in different differentiation, tumor size, mass numbers, and envelope. uPA in HCC with cirrhosis subgroup was higher than no cirrhosis one. uPAR levels were not significantly different in all above subgroup. Plasma TF, uPA and uPAR levels were all related with the invasive and metastatic factor, they were all significantly increased in extrahepatic metastasis, lymphatic metastasis and portal venous tumor thrombus subgroups (P <0.05). Conclusions] Coagulation and anticoagulation were activated in HCC. It indicated that TF, uPA, uPAR might play an important role in hepatic carcinogenesis, invasiveness and metastasis.