脑组织特异表达胆囊收缩素转基因小鼠的建立

目的建立在脑内特异表达胆囊收缩素(CCK)转基因小鼠,研究CCK表达在中枢对饮食行为和代谢的影响。方法构建PDGF-CCK表达载体,利用显微注射法将目的片断注射到受精卵的雄原核中,建立CCK转基因小鼠。通过PCR的方法鉴定转基因鼠的基因型。采用Western Blotting方法鉴定CCK在脑内的表达。用试剂盒测定小鼠血脂并进行统计学分析。结果得到了3个CCK转基因品系,其中2个转基因品系脑内CCK表达量比野生鼠明显增加。高表达CCK的转基因小鼠在正常饲养的情况下体重低于阴性鼠,但血脂未发现显著差异。结论脑内高表达CCK可在一定程度上导致小鼠体重减轻。

The Establishment of Brain Tissue Specific Cholecystokinin Gene Transgenic Mice

Objective To prepare Cholecystokinin(CCK) transgenic mice and investigate the effect of CCK on food intake and metabolism in mice.Methods Transgenic mice were created by the method of microinjection.The genotype of transgenic lines was identified by PCR.The brain specific expression of the CCK gene was analyzed by Western Blotting.The plasma cholesterol and plasma triglyceride were detected and analyzed with a detection kit.Results Three founders of PDGF-CCK transgenic mice were established.Two of PDGF-CCK transgenic independent lines exhibited high-level brain-specific transgene expression compared with that of no-transgenic littermate controls by Western Blotting.The Body weight of the transgenic mice was lower than that of no-transgenic littermate controls,while the plasma cholesterol and plasma triglyceride were not detected obviously different from the controls.Conclusion High expression of CCK in the mouse brain can decrease the action of food intake and decrease body weight of the transgenic mice.