| Cramp过表达对小鼠骨髓造血干细胞功能的影响 |
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| 引用本文： | 石桂英,陈显达,陈陟阳,马小茗,鞠振宇.Cramp过表达对小鼠骨髓造血干细胞功能的影响[J].中国比较医学杂志,2012,22(1):13-17. |
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| 作者姓名： | 石桂英 陈显达 陈陟阳 马小茗 鞠振宇 |
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| 作者单位： | 中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费[DWS201010] |
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| 摘 要： | 目的 研究Cramp蛋白过表达对小鼠骨髓造血干细胞自我更新和分化能力的影响.方法 应用流式细胞仪分析Cramp过表达转基因小鼠及同龄野生型小鼠的骨髓、脾脏、胸腺等组织器官中各种细胞的比例；分选 骨髓造血干细胞,体外培养,观察其克隆形成能力.结果 与野生型小鼠相比,Cramp过表达转基因小鼠的骨髓、脾脏、胸腺等组织器官中各种细胞的比例、骨髓造血干细胞的克隆形成能力等均无明显变化.结论 本研究中,Cramp过表达转基因小鼠骨髓造血干细胞的分化能力、克隆形成能力无明显变化.
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| 关 键 词： | Cramp过表达 转基因小鼠 骨髓造血干细胞 |
Influence of Cramp Over Expression on the Function of Hematopoietic Stem Cell |
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| SHI Gui-ying
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CHEN Xian-da
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CHEN Zhi-yang
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MA Xiao-ming
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JU Zhen-yu.Influence of Cramp Over Expression on the Function of Hematopoietic Stem Cell[J].Chinese Journal of Comparative Medicine,2012,22(1):13-17. |
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| Authors: | SHI Gui-ying CHEN Xian-da CHEN Zhi-yang MA Xiao-ming JU Zhen-yu |
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| Affiliation: | (Key Laboratory of Human Diseases Comparative Medicine,Ministry of Health;Institute of Medical Laboratory Animal Science,Chinese Academy of Medical Sciences;Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine;Peking Union Medicine College,Beijing 100021,China) |
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| Abstract: | Objective to study the role of Cramp gene in the self-renew and differentiation of hematopoietic stem cells(HSCs).Methods cells from the bone marrow,spleen and thymus were analyzed by flow cytometry.HSCs were sorted and cultured in vitro.Results Compared with wild type mice,there are no great differences in the cell percentage and function of bone marrow,spleen and thymus in Cramp over expression mice.Conclusions Compared with the wild type mice,there was no great difference in the HSCs` differentiation and single cell clone in Cramp over expression transgenic mice. |
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| Keywords: | Cramp over expression Transgenic mouse Hematopoietic stem cells |
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