Effect of Sodium Tanshinone ⅡA Sulfonate on Phosphorylation of Extracellular Signal-regulated Kinasel/2 in Angiotensin Ⅱ-induced Hypertrophy of Myocardial Cells |
| |
引用本文: | 李树生,冯俊,郑智,梁黔生.Effect of Sodium Tanshinone ⅡA Sulfonate on Phosphorylation of Extracellular Signal-regulated Kinasel/2 in Angiotensin Ⅱ-induced Hypertrophy of Myocardial Cells[J].Chinese Journal of Integrated Traditional and Western Medicine,2008,14(2):123-127. |
| |
作者姓名: | 李树生 冯俊 郑智 梁黔生 |
| |
作者单位: | Department of Emergency Internal Medicine, Tongji Hospital, Huazhong University of Science and Technology, Wuhan (430030), China |
| |
摘 要: | Objective: To observe the effects of sodium tanshinone ⅡA sulfonate (STS) on angiotensin Ⅱ (Ang Ⅱ)-induced hypertrophy of myocardial cells through the expression of phosphorylated extracellular signal-regulated kinase (p-ERK1/2). Methods: In the primary culture of neonatal rat myocardial cells, the total protein content in myocardial cells was determined by coomassie brilliant blue and the protein synthesis rate was measured by 3H]-Leucine incorporation as indexes for hypertrophy of myocardial cells. The expression of p-ERK1/2 was determined using Western blot and immunofluorescence labeling. Results: (1) The total protein and protein synthesis rate increased significantly in contrast to the control group after the myocardial cells were stimulated by Ang Ⅱ (1 μ mol/L) for 24 h; STS markedly inhibited the increment of the total protein level induced by Ang Ⅱ and the syntheses of protein. (2) After pretreatment of myocardial cells with Ang Ⅱ (1 μmol/L) for 5 min, the p-ERK1/2 protein expression was increased, with the most obvious effect shown at about 10 min; pretreatment of myocardial cells with STS at different doses (2, 10, 50μmol/L) for 30 min resulted in obvious inhibition of the expression of p-ERK1/2 stimulated by Ang Ⅱ in a dose-dependent manner. (3) After the myocardial cells were stimulated by AngⅡ (1 μ mol/L), the immunofluorescence of ERK1/2 rapidly appeared in the nucleus. The activation and translocation process of ERK1/2 induced by Ang Ⅱ was blocked distinctly by STS. (Conclusion: STS inhibited the myocardial cell hypertrophy induced by Ang Ⅱ, and the mechanism may be associated with the inhibition of p-ERK1/2 expression.
|
关 键 词: | 心肌肥大 心肌细胞 血管紧缩素Ⅱ 磺酸钠 |
收稿时间: | 4 March 2008 |
Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells |
| |
Authors: | Shu-sheng Li Jun Feng Zhi Zheng and Qian-sheng Liang |
| |
Affiliation: | (1) Department of Emergency Internal Medicine, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, 430030, China |
| |
Abstract: | Objective To observe the effects of sodium tanshinone II A sulfonate (STS) on angiotensin II (Ang II)-induced hypertrophy of myocardial
cells through the expression of phosphorylated extracellular signal-regulated kinase (p-ERK1/2).
Methods In the primary culture of neonatal rat myocardial cells, the total protein content in myocardial cells was determined by coomassie
brilliant blue and the protein synthesis rate was measured by 3H]-Leucine incorporation as indexes for hypertrophy of myocardial
cells. The expression of p-ERK1/2 was determined using Western blot and immunofluorescence labeling.
Results (1) The total protein and protein synthesis rate increased significantly in contrast to the control group after the myocardial
cells were stimulated by Ang II (1 μmol/L) for 24 h; STS markedly inhibited the increment of the total protein level induced
by Ang II and the syntheses of protein. (2) After pretreatment of myocardial cells with Ang II (1 μmol/L) for 5 min, the p-ERK1/2
protein expression was increased, with the most obvious effect shown at about 10 min; pretreatment of myocardial cells with
STS at different doses (2, 10, 50 μmol/L) for 30 min resulted in obvious inhibition of the expression of p-ERK1/2 stimulated
by Ang II in a dose-dependent manner. (3) After the myocardial cells were stimulated by Ang II (1 μmol/L), the immunofl uorescence
of ERK1/2 rapidly appeared in the nucleus. The activation and translocation process of ERK1/2 induced by Ang II was blocked
distinctly by STS.
Conclusion STS inhibited the myocardial cell hypertrophy induced by Ang II, and the mechanism may be associated with the inhibition of
p-ERK1/2 expression. |
| |
Keywords: | sodium tanshinone II A sulfonate angiotensin II myocardial cell hypertrophy extracellular signal-regulated kinase |
本文献已被 维普 PubMed SpringerLink 等数据库收录! |
| 点击此处可从《Chinese Journal of Integrated Traditional and Western Medicine》浏览原始摘要信息 |
|
点击此处可从《Chinese Journal of Integrated Traditional and Western Medicine》下载全文 |