uPA、uPAR在上皮性卵巢癌的表达及与MMPs相关性研究

目的:探讨尿激酶型纤溶酶原激活物(uPA)及其受体(uPAR)在上皮性卵巢癌组织中的表达意义,研究uPA、uPAR与MMPs在肿瘤侵袭、转移中的相互关系。方法:采用免疫组化S-P法检测100例上皮性卵巢癌原发灶、30例相应的转移灶和20例正常卵巢组织中uPA、uPAR的表达;采用免疫荧光双染法检测uPA、uPAR与MMP-2、MMP-9在上皮性卵巢癌组织中的联合表达。结果:①uPA、uPAR在大多数上皮性卵巢癌原发灶及转移灶组织中均高表达,而在正常卵巢组织中不表达。②uPA和uPAR的高表达与肿瘤分化程度、临床分期、腹水及肿瘤复发显著相关(P<0.01),而与组织学类型及术后残留肿瘤无关(P>0.05)。③uPA、uPAR与MMPs在上皮性卵巢癌原发灶及转移灶组织中均联合表达。结论:uPA和uPAR在上皮性卵巢癌的发展和转移过程中起重要作用;uPA、uPAR与MMPs的相互作用可以促进肿瘤的生长与侵袭;uPA、uPAR可成为晚期、复发卵巢癌治疗的靶向目标。

Expression of uPA and uPAR in epithelial ovarian cancer and relationship between uPA/uPAR and MMPs

Objective:To investigate the expression of uPA and uPAR in epithelial ovarian cancer (EOC) and the relationship between uPA/uPAR and MMPs.Methods:Expression of uPA and uPAR was detected using immunohistochemical staining (SP) in EOC patients(n=100),and matched metastatec lesions (n=30) and normal ovaries tissues (n=20) from untreated patients.Co-immunolabelling of uPA/uPAR and MMP-2、MMP-9 in EOC tissues was detected using immunofluorescence staining.Results:① High expression of uPA and uPAR were observed in primary tumors and metastatic lesions in EOC,but not in normal ovarian tissues.② Over expression of uPA/uPAR in EOC was correlated significantly with progression parameters including tumor grade,relapse and ascites (P<0.01),not correlated with histological type or residual tumor following surgery (P>0.05).③ Co-immunolabeling of uPA/uPAR and MMP was found in EOC specimens,but not in normal ovarian tissues.Conclusion:The results indicated that uPA and uPAR play an key role in EOC progression and metastasis.The interactions of uPA/uPAR and MMPs may promote EOC growth and invasion,which suggested that uPA/uPAR as potential therapeutic targets for treating late-stage recurrent metastatic EOC.