慢性乙肝患者血浆中C_（3）d和SC_（5b－9）的检测及其意义

目的：探讨慢性乙肝患者血浆Ｃ３ｄ和ＳＣ５ｂ－９变化的意义。方法：以ＥＬＩＳＡ双抗夹心法测定３６例ＣＰＨ患者、３９例ＣＡＨ患者及正常对照组血浆Ｃ３ｄ和ＳＣ５ｂ－９含量，并测定补体系统活化的其他有关指标。结果：①ＣＰＨ及ＣＡＨ患者Ｃ３ｄ含量及Ｃ３ｄ／Ｃ３的比值均大于正常对照组，ＣＨ５０、Ｃ３、Ｃ４和ＢＦ水平均小于正常对照组，ＣＡＨ患者ＳＣ５ｂ－９含量大于正常对照组。②ＣＡＨ患者Ｃ３ｄ、ＳＣ５ｂ－９含量及Ｃ３ｄ／Ｃ３的比值均大于ＣＰＨ患者，ＣＨ５０含量小于ＣＰＨ患者。③ＣＡＨ患者ＳＣ５ｂ－９与Ｃ３ｄ、ＣＨ５０分别是正、负相关，且与ＡＬＴ水平呈正相关。结论：测定血浆Ｃ３ｄ、ＳＣ５ｂ－９含量及Ｃ３ｄ／Ｃ３的比值有助于临床上对慢性乙肝病情和预后作出判断。进一步了解ＭＡＣ在肝细胞的存在情况，有助于加深对慢性乙肝发病机理的认识。

Detection and Signification of C_(3)d and SC_(5b-9) in Plasma of Chronic Hepatitis B Patients

To study the effects of changes in C3d and SC5b-9 levels in plasma of patients with chronic hepatitis B. METHODS:Sandwich ELISA was used to measure levels of C3d,SC5b-9 and several indexes related to complement system activation were examined in plasma of 36 patients with chronic persistent hepatitis (CPH), 39 patients with chronic active hepatitis (CAH),and normal subjects. RESULTS: 1. The levels of C3d and C3d/C3 ratio of CPH and CAH were higher than those of the normol control. The levels of CH50,C3,C4 and BF were lower than those o f the normal control group. The levels of SC5b-9 of CAH were higher than those of the normol control group. 2. The levels of C3d, SC5b-9 and C3d/C3 ratio of CAH were higher than those of CPH,but levels of CH50 were lower than those of CPH. 3. SC5b-9, level showed positive correlation with C3d and ALT level,negative correlation with CH50 in patients with CAH. CONCLUSIONS:The results suggest that the levels of C3d,SC5b-9 and C3d/C3 ratio in plasma may be of great help in diagnosing condition and predicting prognosis in patients with chronic hepatitis B. Further, the distribution of membrane attack complex in liver tissue is helpful in recognizing the pathogenesis of chronic hepatitis.