| nm23H1正反义表达载体的构建 |
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| 引用本文： | 金嗣松,潘星华,王元和,胡以平,傅继梁.nm23H1正反义表达载体的构建[J].第二军医大学学报,1999,20(6):350-352. |
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| 作者姓名： | 金嗣松 潘星华 王元和 胡以平 傅继梁 |
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| 作者单位： | 1. 第二军医大学长征医院普通外科
2. 第二军医大学基础医学部生物学教研室,上海,200433 |
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| 摘 要： | 目的；构建ｎｍ２３Ｈ１正反义表达载体ｐｃ３ＡＮ和ｐｃ３ＡＭ。方法：将ｎｍ２３Ｈ１基因正，反向插入质粒ｐｃＤＮＡ３和ｐＲＣ／ＣＭＶ，并转染肝癌细胞ＳＭＭＣ７７２１。结果：转染正义表达载体后ｎｍ２３Ｈ１ｍＲＮＡ和蛋白表达水平增加，转染反义表达载体后ｎｍ２３Ｈ１ｍＲＮＡ和蛋白表达水平降低。结论：构建的载体能在肝癌细胞内有效表达。
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| 关 键 词： | 基因 表达载体 肝肿瘤 |
Construction of expression vector of sense and antisense nm23H1 |
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| Jin Sisong,Pan Xinghua,Wang Yuanhe,Hu Yiping,Fu Jiliang.Construction of expression vector of sense and antisense nm23H1[J].Academic Journal of Second Military Medical University,1999,20(6):350-352. |
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| Authors: | Jin Sisong Pan Xinghua Wang Yuanhe Hu Yiping Fu Jiliang |
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| Abstract: | Objective: To construct expression vector of sense and antisense nm23H1: pc3AN and pc3AM. Methods: nm23H1 gene was inserted into plasmid pcDNA3 and pRC/CMV in proper sequence and in reverse sequence, the production of above action was used to transfect liver cancer cell SMMC7721. Results: The level of mRNA and protein of nm23H1 was high when transfected with sense vector, and was low when transfected with antisense vector. Conclusion: The constructed vectors can highly express in liver cancer cell. |
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| Keywords: | nm23H1 |
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