三种药物对大鼠糖尿病性白内障延缓效应的研究

目的 本实验旨在验证腹腔注射葡萄糖能否成功建立大鼠糖性白内障模型，并且探讨黄芩苷点眼、牛磺酸点眼、牛磺酸腹腔注射以及Vc腹腔注射对大鼠糖性白内障的延缓效应及短期治疗作用。方法 本实验按照公鼠2.5ml/次，母鼠2ml/次剂量注射30%葡萄糖生理盐水建立大鼠糖性白内障模型，给药同期进行，点眼每日2次，腹腔注射每日1次。从注射葡萄糖第3日开始对各组晶状体进行裂隙灯检查，并定期测定体重、饮食量等相关指标。结果 实验结果表明葡萄糖腹腔注射第3天即可使大鼠晶状体出现空泡现象。各组药物的延缓效应与短期治疗效应相同，依次为牛磺酸注射、黄芩苷点眼、牛磺酸点眼以及Vc注射。结论 30%葡萄糖腹腔注射能快速建立大鼠糖性白内障模型，且比半乳糖注射建模成本低，成模率高。4%牛磺酸腹腔注射以及0.1%黄芩苷在预防和治疗大鼠糖性白内障方面效果显著。

The comparative study of delay effect of three drugs on diabetic cataract of rats

Objective To examine whether glucose intraperitoneal injection could successfully establish rat sugar cataract models. We also discussed the delay effect and short-term therapeutic effect of Baicalin eyedrop, taurine eyedrop, taurine intraperitoneal injection and Vc intraperitoneal injection on cataract models. Methods The study established cataract models using intraperitoneal injection of 30% glucose following the doses of 2.5ml/time for a male rat and 2ml/time for a female rat. We also provided all drugs at the same time, including eyedrops for twice a day, and intraperitoneal injection for once a day. We examined lens situation using slit-lamp from the third day of injection. We also measured body weight and diet situation during the study. Results The study showed that glucose intraperitoneal injection could establish sugar cataract in 3 days. The delay effect and short-term therapeutic effect of every drug had the same trandency, and they were taurine intraperitoneal injection > Baicalin eyedrop > taurine eyedrop > Vc intraperitoneal injection. Conclusions 30% Glucose intraperitoneal injection could induce rat sugar cataract in a short period. Further more, the delaying effect of taurine injection and Chinese traditional medicine Baicalin eyedrop were more obvious compared with taurine eyedrop and Vc injection.