表型分析在PML-RARA转基因小鼠筛选中的应用

PML－RARA转基因（Tg)小鼠发生急性早幼粒细胞白血病（APL），为深入研究APL发病机理提供了良好模型。对Tg小鼠进行定期观察和表型分析，结果表明PML－RARA Tg小鼠经历白血病前期和白血病早期阶段，最终发展成典型的终末期白血病，证实Tg小鼠APL的发生是一个经历多阶段的渐进过程，各阶段具有明确可分的细胞形态学和组织病理学特征性改变。通过对Tg小鼠进行定期观察并作外周血、骨髓象和组织病理学分析，筛选处于APL不同发病阶段的Tg小鼠，为研究APL多阶段渐进的发病过程中基因型改变提供了良好的实验依据。

Screening of the PML-RARA Transgenic Mice by Using Phenotypic Analysis

PML-RARA transgenic(Tg) mice developed acute promyelocytic leukemia(APL) with APL features which provided us with a powerful model to further our study on the mechanism of APL. Tg mice were observed and peripheral blood(PB) analysis was done at scheduled time. Tg mice of different ages were picked up and sacrificed. Phenotypic analysis showed that the course of APL development could be divided mainly into three stages: pre-leukemic stage ,early leukemia stage and advanced leukemia stage. Phenotypic features which is specific for each stage could be observed. Thus, screening of Tg mice by using phenotypic analysis (cytologic analysis of PB and bone marrow(BM) ,histo-pathology of BM)make it possible for us to pick up the Tg mice at those different stages which is crucial for the study of genetic changes during the development of APL.