| 甲型H1N1流感病毒在季流病毒、禽流感病毒共感染时变异可能性的验证 |
| |
| 引用本文： | 鲍琳琳,许黎黎,孙惠惠,占玲俊,邓巍,朱华,高虹,陈鸿霖,秦川.甲型H1N1流感病毒在季流病毒、禽流感病毒共感染时变异可能性的验证[J].实验动物与比较医学,2011,19(3). |
| |
| 作者姓名： | 鲍琳琳 许黎黎 孙惠惠 占玲俊 邓巍 朱华 高虹 陈鸿霖 秦川 |
| |
| 作者单位： | 1. 中国医学科学院实验动物研究所,国家中医药管理局人类疾病动物模型三级实验室,卫生部人类疾病2. 香港大学微生物系新发传染病国家重点实验室,香港 |
| |
| 基金项目： | 科技重大专项-艾滋病和病毒性肝炎等重大传染病防治，卫生公益性行业科研专项项目，中央级公益性科研院所基本科研业务费 |
| |
| 摘 要： | 目的 甲型H1N1流感病毒A/California/7/2009分别与A/Brisbane/10/07和A/ShenZhen/406H/06共感染小型香猪,预测甲流病毒在与季流H3N2病毒/甲流病毒与禽流感病毒共感染时是否会发生变异.方法 分别将A/California/7/2009(CA7)与A/Brisbane/10/07(H3N2),A/California/7/2009与A/Shenzhen/406H/06(H5N1)对5～6月龄小型猪共感染,小型猪经复方氯胺酮0.1 mL/kg麻醉后进行滴鼻感染,感染后第5天安乐死动物,取动物肺组织作病毒测序分析.结果 A/California/7/2009(CA7)与A/Brisbane/10/07(H3N2)共感染后,A/California/7/2009病毒PB1基因993位G→A突变,PA基因1659位G→A突变,没有氨基酸的变异.A/California/7/2009与A/Shenzhen/406H/06(H5N1)共感染后A/California/7/2009病毒PB2基因1711位T→C突变.碱基的突变未引起氨基酸的变异.结论 A/California/7/2009(CA7)与A/Brisbane/10/07(H3N2),A/California/7/2009与A/Shenzhen/406H/06(H5N1)共感染后在猪的体内没有发生病毒重组、变异.
|
| 关 键 词： | 小型猪 变异 |
No mutation of influenza A (H1N1) virus at co-infection with seasonal influenza and avian influenza viruses in miniature pigs |
| |
| BAO Lin-lin,XU Li-li,SUN Hui-hui,ZHAN Ling-jun,DENG Wei,ZHU Hu,GAO Hong,CHEN Hong-lin,QIN Chuan.No mutation of influenza A (H1N1) virus at co-infection with seasonal influenza and avian influenza viruses in miniature pigs[J].Laboratory Animal and Comparative Medicine,2011,19(3). |
| |
| Authors: | BAO Lin-lin XU Li-li SUN Hui-hui ZHAN Ling-jun DENG Wei ZHU Hu GAO Hong CHEN Hong-lin QIN Chuan |
| |
| Affiliation: | BAO Lin-lin1,XU Li-li1,SUN Hui-hui1,ZHAN Ling-jun1,DENG Wei1,ZHU Hua1,GAO Hong1,CHEN Hong-lin2,QIN Chuan1(1.Center of Comparative Medicine,Institute of Laboratory Animal Science,CAMS & PUMC,Key Laboratory of Human Diseases Animal Model,State Administration of Traditional Chinese Medicine,Key Laboratory Animal of Human Diseases Comparative Medicine,Ministry of Health,Beijing 100021,China,2.State Key Laboratory of Emerging Infectious Diseases,Department of Microbiology,The University of Hong Kong,Pokfulam... |
| |
| Abstract: | Objective To co-infect miniature pigs with influenza A virus A/California/7/2009 with A/Brisbane/10/07 and A/Shenzhen/406H/06 virus,respectively,and to verify whether the co-infection of A/California/7/2009(CA7) and A/Brisbane/10/07(H3N2) or A/California/7/2009 and A/Shenzhen/406H/06(H5N1) influenza viruses will induce mutation in influenza virus.Methods To co-infect the 5-6 month old miniature pigs with A/California/7/2009(CA7) and A/Brisbane/10/07(H3N2) or A/California/7/2009 and A/Shenzhen/406H/06(H5N1) ... |
| |
| Keywords: | A/California/7/2009 A/Brisbane/10/07 A/Shenzhen/406H/06 |
|
| 点击此处可从《实验动物与比较医学》浏览原始摘要信息 |
|
点击此处可从《实验动物与比较医学》下载全文 |
|
