一个灵长类种属特异基因——XAGE-3转基因

目的比较研究F344fNSIc与WistarNWN两种大鼠对1．溴丙烷（1-BP）引发的毒性发应。方法雄性WistarNWN和F344／NSIc大鼠各6只，吸入染毒5030mg／m31-BP1周。取睾丸做病理切片，过碘酸希夫氏染色；切取胫后支神经，Kliiver．Barrera法染色，光镜下观察。结果睾丸组织病理学显示，1．BP染毒后第VIII发育阶段的生精上皮中出现长形精子细胞滞留。胫后支神经组织病理学显示，1-BP染毒后周围神经的髓鞘出现卵圆形的缺失变性。Wistar和F344fNSIc两种大鼠均出现了上述病理学改变。结论1-BP毒性的典型病理学改变在F344／NSIc与WistarNWN两种大鼠中均得以重现。F344／NSIc纯种大鼠是进行1-溴丙烷毒效应基因水平的研究的适宜品系。

XAGE-3,A Primate Specific Gene Transgenic Research

Objective To study the 1-Bromopropane （1-BF） induced toxic effects in F344/NSIc rat and Wistar NWN rat. Methods Six male Wistar NWN and six male F344/NSIc rats were exposed to 5030 mg/m3 1-bromopropane by inhalation for continuously 1 week. Animals were sacrificed, and the testes and posterior tibial nerve were dissected for histopathological examination, either stained by periodic acid-Schiff staining method or stained by Kliiver-Barrera staining method. Results Testicular histopathology showed that retained, enlongated sperms were found in the seminiferous epithelium （stage VIII） in both strains, which indicated the spermiation failure. Pathology of posterior tibial nerve showed that ovoid or bubble-like debris of myelin sheath was also found in both F344/NSIc and Wistar NWN rats. Conclusion The typical pathological change of 1-BP induced toxicity can be reproduced in both F344/NSIc and Wistar NWN rats. F344/NSIc in-bred rat is the suitable strain for studying the toxic effects of 1-BP at gene level.