PELGE纳米粒的制备及影响粒径大小的因素

目的通过开环共聚方法合成三嵌段共聚物(PEG—PLGA—PEG，简称PELGE)。采用超声乳化一溶剂蒸发法(O／W)制备PELGE纳米粒。方法对可能影响纳米粒粒径大小的因素，如有机相和水相的体积比、聚合物的浓度、表面活性剂的浓度等做了较详细的考察。结果其优选方案为：高分子载体材料的质量浓度为10mg／ml，有机溶剂为丙酮／二氯甲烷(体积比为2／3/a)的混合溶剂，F68溶液的质量浓度为30g／L，油相与水相体积比为1：8。结论制备的纳米粒大小均匀，呈规整球形，粒径分布范围为60～100nm。

The Preparation of PELGE-NP and a Study of the Factors Affecting Their Diameter

Objective To prepare Poly(ethylene glycol) - poly (lacticacid-co-glycolicacid)- poly(ethylene- glycol) nanoparticles (PELGE-NP) and investigate the factors affecting their diameter. Methods PELGE were synthesized by ring-opening polymerization, PELGE nanoparticles(PELGE-NP) were prepared by using the emulsion-solvent evaporation technique (O/W). Orthogonal design was applied to optimize the preparation technology on the basis of the single factor evaluation. Results The optimal conditions for preparing nanoparticles included the PELGE at concentration of 10 mg/ml, the ratio of acetone/DCM at 2/3, the F68 at concentration of 3%, and the volume ratio of O/W at 1/8(V/V). Conclusion The prepared PELGE nanoparticles are spherical and discrete particles without aggregation; they are smooth in surface morphology, and their diameters range from 60 nm to 100 nm.