黄连素对实验性再狭窄模型血管内皮功能的影响

目的:探讨黄连素对兔颈动脉球囊损伤后血一氧化氮(NO)、内皮素-1(ET-1)水平及血管形态改变的影响,并初步探讨其改善血管内皮功能的可能机制。方法:将40只日本大耳白兔,随机分为假手术组、模型组、黄连素组和辛伐他汀组,除假手术组外各组均以球囊导管扩张损伤颈动脉内膜,并分别给予生理盐水、黄连素注射液和辛伐他汀配制液2.5 mg/(kg·d)腹腔注射,假手术组仅普食喂养。分别于术后3、7及15天探测各组右颈总动脉直径,并于耳缘静脉采血,检测血清NO含量及血浆ET-1浓度;取15天时的颈动脉切片于光镜下观察。结果:术后3、7及15天,黄连素组血清NO含量与假手术组无明显差异,但显著高于模型组及辛伐他汀组(P0.05);黄连素组和辛伐他汀组血浆ET-1水平与假手术组均无明显差异,但显著低于模型组(P0.05);术后15天,黄连素组和辛伐他汀组血管直径较假手术组均无明显差异,但显著大于模型组(P0.05);黄连素和辛伐他汀组新生内膜中的平滑肌细胞较同期模型组明显减少(P0.05),泡沫细胞、纤维结缔组织较少,内皮较完整光滑,内膜增生较轻。结论:黄连素可能通过抑制兔颈动脉球囊损伤后NO浓度降低和ET-1水平升高,抑制新生内膜增生、改善血管内皮功能。

Influence of Berberine on Venous Endothelial Function of Experimental Model of Restenosis

Objective: To explore the possible mechanism of berberine improving venous endothelial function initially through discussing its influence on the levels of nitric oxide(NO) and endothelin-1 (ET-1) of the rabbits with carotid artery balloon injury. Methods: Forty rabbits were randomly allocated to sham operation group, the model group, berberine group and simvastatin group, except sham operation group, the rabbit models in other groups were prepared by balloon catheter dilation injuring carotid artery intima, and they accepted peritoneal injection of physio-logical saline, berberine injection and simvastatin preparation solution in the concentrations of 2.5 mg/(kg·d), sham operation group were fed with common food. The diameters of right common carotid artery in different groups were measured in three, seven and 15 days after the operations, and blood was drawn from auricular vein, the contents of NO and the concentrations of ET-1 were detected; carotid artery slice in 15 days after the operation was observed un-der light microscope. Results: In three, seven and 15 days after the operations, there was no significant difference be-tween the berberine group and sham operation group in the contents of No, but the berberine group was higher than the model group and simvastatin group significantly (P<0.05); No significant difference was existed in the compari-son among the berberine group, simvastatin group and sham operation group in the levels of ET-1, but the berberine group and simvastatin group were lower than the model group remarkably in the levels of ET-1 (P<0.05); in 15 days after the operation, there was no significant difference in the comparison among the berberine group, simvastatin group and sham operation group in vessel diameter, the berberine group and simvastatin group were larger than the model group in vessel diameter(P<0.05); smooth muscle cells decreased in new intima in the berberine group and simvastatin group compared with the model group at the same time obviously(P<0.05), foam cells and fibrous con-nective tissue were less, endothelium was complete and smooth, endometrial hyperplasia was more light. Conclu-sion: Berberine could inhibit new endometrial hyperplasia and improve vascular endothelial function by decreasing the concentrations of NO and raising the level of ET-1 after inhibiting rabbit carotid artery balloon injury.