不同产地葫芦巴提取物降血糖活性评价

目的研究不同产地葫芦巴提取物对正常小鼠及四氧嘧啶所致高血糖小鼠的降血糖作用。方法①将健康小鼠90只随机分为云南产葫芦巴低、高剂量组,甘肃产葫芦巴低、高剂量组,河北产葫芦巴低、高剂量组,河南产葫芦巴低、高剂量组和空白对照组,各10只。空白对照组给予等容积纯化水,其他各组低、高剂量组分别给予200、400mg/（kg.d）相应产地葫芦巴提取物,称体质量并测空腹血糖值。然后再灌胃给予葡萄糖溶液2g/（kg.d）,测定葡萄糖负荷后血糖值并计算血糖曲线下面积（AUC）。②将健康小鼠100只腹腔注射四氧嘧啶,测空腹血糖;随机分为云南产葫芦巴低、高剂量组,甘肃产葫芦巴低、高剂量组,河北产葫芦巴低、高剂量组,河南产葫芦巴低、高剂量组,模型组和阳性药对照组,各10只。模型组给予等容积纯化水,阳性药对照组给予盐酸苯乙双胍片40mg/（kg.d）,其他各组低、高剂量组分别给予200、400mg/（kg.d）相应产地葫芦巴提取物,测空腹血糖值。然后再灌胃给予葡萄糖溶液2g/（kg.d）,测定葡萄糖负荷后血糖值,并计算AUC。结果不同产地葫芦巴提取物对正常小鼠体质量、空腹血糖以及葡萄糖负荷后各时间点空腹血糖值和AUC均无显著影响。云南、河北产葫芦巴提取物低、高剂量组和甘肃、河南产葫芦巴高剂量组对四氧嘧啶致高血糖小鼠均可显著降低空腹血糖水平,且可显著降低葡萄糖负荷后不同时间点小鼠的空腹血糖值,减少AUC。结论不同产地葫芦巴提取物高剂量组均可一定程度降低四氧嘧啶所致高血糖小鼠的空腹血糖,且对其糖耐量有一定调节作用。

Research on the hypoglycemic effect of Frenugreek extracts from different producing area

Objective To evaluate the hypoglycemic effects of the extracts from Frenugreek of different producing area on normal mice and alloxan-induced diabetic mice.Methods ①90 mice were randomly divided into nine groups,low-,high-dose Frenugreek extracts groups from Henan,low-,high-dose Frenugreek extracts groups from Hebei,low-,high-dose Frenugreek extracts groups from Henan and control group.Then they were administrated with the extracts（200,400 mg/kg·d）from different producing area,mice in control group were administrated with isovolumetric purified water.The body weight and FBG were measured.Blood glucose was measured after oral administration with glucose liquid（2 g/kg·d）,and the change of area under the blood glucose curve（AUC）was calculated.②100 diabetic mice were induced by alloxan in Kunming mice.FBG were measured.And they were randomly divided into ten groups.Then they were administrated with the extracts（200,400 mg/kg·d）from different producing area,mice in control group were administrated with isovolumetric purified water.Mice in positive control group were administrated with Phenformin hydrochloride tablets,40 mg/kg·d.The changes of FBG value were observed.Blood glucose was measured after oral administration with glucose liquid（2 g/kg·d）,and the change of area under the blood glucose curve（AUC）was calculated.The same method was used to evaluate the influence of extracts on body weight,blood glucose value and glucose tolerance in normal mice.Results The extracts had no influence on body weight,blood glucose value and the AUC induced by the glucose loading in normal mice.The Frenugreek extracts from Yunnan and Hebei decreased the level of blood glucose in different sense respectively on diabetic model.The blood glucose value of 0,0.5,2 hours and the AUC induced by the glucose loading were decreased as well.Conclusion The extracts of Frenugreek from different producing area have the functions of lowering the blood glucose and regulating glucose tolerance on alloxan-caused diabetic mice.