| 桂枝加葛根汤对椎间盘纤维环细胞蛋白多糖表达的影响 |
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| 引用本文： | 王诗忠,黄龙生,陈少清,廖军,仲卫红,宋红梅,林久茂.桂枝加葛根汤对椎间盘纤维环细胞蛋白多糖表达的影响[J].中华中医药杂志,2011(11):2543-2547. |
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| 作者姓名： | 王诗忠 黄龙生 陈少清 廖军 仲卫红 宋红梅 林久茂 |
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| 作者单位： | 福建中医药大学;福建中医药大学附属第二人民医院康复分院;国家中医药管理局中医康复技术三级实验室; |
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| 基金项目： | 国家自然科学基金(No.30973764); 福建省卫生教育联合攻关计划项目(No.WKJ2008-2-48); 福建省科技厅国际合作项目(No.2009I0010),福建省科技厅青年人才项目(No.2008F3052)~~ |
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| 摘 要： | 目的:探讨颈椎病的发病机制及桂枝加葛根汤防治该病的作用机制。方法:采用自制桂枝加葛根汤大鼠含药血清,对培养的椎间盘纤维环细胞进行不同浓度的药物干预,利用荧光定量PCR检测椎间盘纤维环中主要蛋白多糖Aggrecan mRNA的表达变化情况。结果:中、高剂量组Aggrecan mRNA表达的Ct值明显高于空白组(P<0.05),低剂量组Ct值与空白组比较无明显差异,而高剂量组Ct值明显高于中剂量组(P<0.05)。结论:中、高剂量组桂枝加葛根汤大鼠含药血清能增强纤维环细胞Aggrecan mRNA的表达,高剂量组效果最为明显。桂枝加葛根汤从转录水平上影响纤维环细胞蛋白多糖的产生。
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| 关 键 词： | 颈椎病 桂枝加葛根汤 纤维环细胞 |
Effects of Guizhi plus Gegen Decoction on expression of proteoglycan in intervertebral disc annulus fibrosus cells |
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| WANG Shi-zhong,,HUANG Long-sheng,CHEN Shao-qing,LIAO Jun,ZHONG Wei-hong,SONG Hong-mei,LIN Jiu-mao.Effects of Guizhi plus Gegen Decoction on expression of proteoglycan in intervertebral disc annulus fibrosus cells[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(11):2543-2547. |
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| Authors: | WANG Shi-zhong HUANG Long-sheng CHEN Shao-qing LIAO Jun ZHONG Wei-hong SONG Hong-mei LIN Jiu-mao |
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| Affiliation: | WANG Shi-zhong1,2,3,HUANG Long-sheng1,CHEN Shao-qing1,LIAO Jun1,ZHONG Wei-hong2,SONG Hong-mei2,LIN Jiu-mao1 (1Fujian University of TCM,Fuzhou 350108,China,2Rehabilitation Branch of The Secend People's Hospital,Fujian University of TCM,Fuzhou 350003,3The Third Grade Laboratory of Recovery Technology of TCM,State Administration of TCM,China) |
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| Abstract: | Obiective: To explore the pathogenesis of cervical syndrome and the mechanisms of Guizhi plus Gegen Decoction(GGD) in preventing cervical syndrome.Methods: Intervertebral disc annulus fibrosus cells were interfered whih different concentrations of drug that made by rats serum which contained GGD.Then real-time quantitative PCR was used to detect changes of aggrecan mRNA which was the main proteoglycan in intervertebral disc annulus fibrosus cells.Results: The Ct value of aggrecan mRNA expression in middle-d... |
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| Keywords: | Cervical syndrome Guizhi plus Gegen Decoction Annulus fibrosus cells |
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