| 健脾解毒方对脾虚证荷瘤小鼠肠道菌群的调节作用 |
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| 引用本文： | 陈彬,孙珏,梁芳,徐可,万光升,袁旭.健脾解毒方对脾虚证荷瘤小鼠肠道菌群的调节作用[J].上海中医药杂志,2018,52(1):81-85. |
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| 作者姓名： | 陈彬 孙珏 梁芳 徐可 万光升 袁旭 |
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| 作者单位： | 上海中医药大学附属普陀医院中医肿瘤科;上海中医药大学中西医结合肿瘤介入研究所; |
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| 基金项目： | 上海中医药大学预算内项目(2015YSN51) |
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| 摘 要： | 目的探讨健脾解毒方对脾虚荷瘤鼠移植瘤的抑制作用、对脾虚小鼠肠道菌群的调节作用及两者之间的相关性。方法 48只小鼠随机取36只,利用番泻叶水煎剂成功制备脾虚模型,剩余12只为N.S.组,再使用CT26细胞构建移植瘤模型。36只脾虚模型小鼠则随机分为脾虚组、健脾解毒方低剂量组、健脾解毒方高剂量组,每组12只。连续灌胃给药4周后脱颈椎处死各组小鼠,称取瘤重,计算抑瘤率;计算胸腺指数和脾指数;流式细胞仪检测CD4~+、CD8~+T细胞比例,ELISA法检测血清中IL-17水平;16S rRNA基因测序法检测肠道菌群多样性。结果脾虚组平均瘤重、血清IL-17水平高于N.S.组和健脾解毒方低、高剂量组,同时脾虚组胸腺指数、脾指数、外周血CD4~+T细胞比例、CD4~+/CD8~+比值低于N.S.组和健脾解毒方低、高剂量组,差异均有统计学意义(P0.01);且脾虚组小鼠肠道菌群中拟杆菌门显著增多、厚壁菌门显著减少,存在明显的菌群失调现象。与N.S.组及健脾解毒方低、高剂量组比较,脾虚组小鼠肠道中双歧杆菌及乳杆菌减少,而肠杆菌及肠球菌增多。结论健脾解毒方具有抑制脾虚荷瘤鼠皮下瘤生长、提高CD4~+/CD8~+比例、减少炎症因子IL-17分泌的作用,这些作用可能与其改善脾虚小鼠的肠道菌群失调相关。
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| 关 键 词： | 健脾解毒方 脾虚 大肠癌 小鼠模型 肠道菌群失调 CD4+/CD8+ IL-17 |
| 收稿时间： | 2017/5/20 0:00:00 |
Regulation of Jianpi Jiedu Recipe on intestinal flora in tumor bearing mice with syndrome of spleen deficiency |
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| CHEN Bin,SUN Jue,LIANG Fang,XU Ke,WAN Guangsheng and YUAN Xu.Regulation of Jianpi Jiedu Recipe on intestinal flora in tumor bearing mice with syndrome of spleen deficiency[J].Shanghai Journal of Traditional Chinese Medicine,2018,52(1):81-85. |
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| Authors: | CHEN Bin SUN Jue LIANG Fang XU Ke WAN Guangsheng and YUAN Xu |
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| Affiliation: | Oncology Department of Chinese Medicine,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China,Oncology Department of Chinese Medicine,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China,Oncology Department of Chinese Medicine,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China,Tumor Intervention Institute of Integrated Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China,Oncology Department of Chinese Medicine,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China and Oncology Department of Chinese Medicine,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China |
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