多指标优化吴茱萸的提取纯化工艺

目的:优选吴茱萸的提取纯化工艺.方法:以吴茱萸内酯、吴茱萸碱、吴茱萸次碱转移率的综合评分为指标,采用正交试验考察溶媒倍数、溶剂浓度、提取次数和提取时间对吴茱萸醇提工艺的影响；以吴茱萸内酯、吴茱萸碱、吴茱萸次碱的质量分数为指标,通过单因素试验考察吴茱萸提取液逐步水沉纯化工艺.结果:最佳提取工艺为加16倍量70％乙醇回流提取3次,每次3h;最佳逐步水沉纯化工艺为回收乙醇,离心,取沉淀加20倍量90％乙醇超声提取15 min,离心,取上清液,加1/4倍量水进行二次水沉.结论:优选的提取、纯化工艺可确保吴茱萸中有效成分最大限度保留.

Optimization of Extraction and Purification Technology of Active Components from Evodia rutaecarpa by Multi-indexes

Objective:To optimize extraction and purification technology of Evodia rutaecarpa. Method: With tranfer rate of evodine,evodiamine and rutacarpine as comprehensive evaluation index,influence of extraction times,solvent multiples,solvent concentration and extraction time on alcohol extraction technology of E. rutaecarpa was investigated by orthogonal test;With the mass fraction of evodine,evodiamine and rutacarpine as indexes,single factor test was used to investigate progressive water precipitation purification technology of extraction solution. Result: Optimum extraction technology was as following:refluxing extracted 3 times with 16 times the amount of 70% ethanol,3 hours each time;optimum progressive water precipitation purification technology:recovery of ethanol,centrifugation,precipitate was ultrasonic extracted 15 min with 20 times the amount of 90% ethanol,centrifugation,taking the supernatant,added quarter times of water in second time of water precipitation. Conclusion: Optimized extraction and purification technology could ensure effective components from E. rutaecarpa to maximize retention.