| 硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较 |
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| 引用本文： | 辛敏,刘轩,詹欣,黄昀,唐劲天,盛军.硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较[J].中国实验方剂学杂志,2014,20(3):62-65. |
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| 作者姓名： | 辛敏 刘轩 詹欣 黄昀 唐劲天 盛军 |
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| 作者单位： | 北京中医药大学中药学院, 北京 100102;清华大学工程物理系粒子技术与辐射成像教育部重点实验室, 北京 100084;清华大学工程物理系粒子技术与辐射成像教育部重点实验室, 北京 100084;北京中医药大学中药学院, 北京 100102;清华大学工程物理系粒子技术与辐射成像教育部重点实验室, 北京 100084;清华大学工程物理系粒子技术与辐射成像教育部重点实验室, 北京 100084;北京中医药大学中药学院, 北京 100102;清华大学工程物理系粒子技术与辐射成像教育部重点实验室, 北京 100084;北京中医药大学中药学院, 北京 100102;云南农业大学普洱茶学教育部重点实验室, 昆明 650201 |
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| 基金项目： | 中国博士后基金项目（2013M530603） |
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| 摘 要： | 目的： 比较苯酚-硫酸法与硫酸-紫外法在测定千两茶多糖含量上的差异。 方法： 千两茶粉经水提醇沉，Sevage法除蛋白后，采用苯酚-硫酸法和硫酸-紫外法测定茶多糖含量。 结果： 苯酚-硫酸法标准曲线方程为Y=4.755 0X-0.029 5（R2=0.990 4），硫酸-紫外法标准曲线方程为Y=4.770 0X-0.065 1（R2=0.993 1），均在0.02~0.1 g·L-1有良好的线性关系。所测茶多糖质量分数分别为2.00%，3.21%，其差异有统计学意义。 结论： 硫酸-紫外法作为一种多糖检测新手段，可简便测定多糖含量。
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| 关 键 词： | 茶多糖 千两茶 苯酚-硫酸法 硫酸-紫外法 含量测定 |
| 收稿时间： | 2013/7/17 0:00:00 |
Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method |
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| XIN Min,LIU Xuan,ZHAN Xin,HUANG Yun,TANG Jin-tian and SHENG Jun.Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method[J].China Journal of Experimental Traditional Medical Formulae,2014,20(3):62-65. |
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| Authors: | XIN Min LIU Xuan ZHAN Xin HUANG Yun TANG Jin-tian and SHENG Jun |
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| Affiliation: | Beijing University of Chinese Medicine, Beijing 100102, China;Institute of Nuclear Energy Technology, Tsinaghua University, Beijing 100084, China;Institute of Nuclear Energy Technology, Tsinaghua University, Beijing 100084, China;Beijing University of Chinese Medicine, Beijing 100102, China;Institute of Nuclear Energy Technology, Tsinaghua University, Beijing 100084, China;Institute of Nuclear Energy Technology, Tsinaghua University, Beijing 100084, China;Beijing University of Chinese Medicine, Beijing 100102, China;Institute of Nuclear Energy Technology, Tsinaghua University, Beijing 100084, China;Beijing University of Chinese Medicine, Beijing 100102, China;Yunnan Research Centre for Advance Tea Processing, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | Objective: To compare differences between phenol-sulfuric acid method and sulfuric acid-UV method to determine polysaccharides content in Qianliang tea. Method: The two methods were employed to determine content of polysaccharides in Qianliang tea after water extracting-alcohol precipitating and sevage assays. Result: The total sugar stand curve equation were Y=4.755 0X-0.029 5(R2=0.990 4)(by sulfuric acid-UV method) and Y=4.770 0X-0.065 1(R2=0.993 1)(by phenol-sulfuric acid method), which showed a good linear relationship range from 0.02 to 0.1 g·L-1. The determination of phenol-sulfuric acid method and sulfuric acid-UV method were 2.00% and 3.21%, which was statistically significance. Conclusion: As a new method, sulfuric acid-UV method could be a stable and simple method to measure the polysaccharide. |
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| Keywords: | tea polysaccharides qianliang tea phenol-sulfuric acid sulfuric acid-UV determination |
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