秦岭柴胡不同提取物保肝作用量效关系研究

目的:探讨两种产地秦岭柴胡不同提取物对D-氨基半乳糖致急性肝损伤小鼠的保护作用,并初步筛选最佳提取工艺和活性部位。方法:将小鼠分为9组:空白对照组,半乳糖胺模型组,联苯双酯滴丸(150 mg.kg-1),秦岭柴胡1号样品水提组、65%醇提组、95%醇提组,秦岭柴胡2号样品水提组、65%醇提组、95%醇提组。剂量为5.0 g.kg-1,灌胃给药,给药体积20 mL.kg-1,模型组和空白组分别灌胃给予同体积生理盐水,每天1次,连续给药7 d后,采取小鼠腹腔注射D-氨基半乳糖盐酸盐溶液造模,16 h后眼静脉采血,测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性;同时测定肝匀浆中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果:2种产地秦岭柴胡的水提组、65%醇提组、95%醇提组均能不同程度降低CCl4模型小鼠血清ALT,AST活性,降低肝脏MDA的含量,增强SOD活性,其中以东太白产秦岭柴胡65%醇提物作用最强(P<0.01,P<0.05)。结论:秦岭柴胡对D-氨基半乳糖盐酸盐所致小鼠急性肝损伤具有较明显的保护作用,其保肝作用与柴胡皂苷a,d含量存在一定的量效关系。

Experimental Study on Dose-effect Relationship of Hepatoprotective Effect of the Different Extraction from Bupleurum longicaule var. giraldii

Objective:To discuss protective effect of different extractions of two origin Bupleurum longicaule var.giraldii on acute liver injury in mice caused by intraperitoneal injection(ip)D-galactosamine, and to screen the optimum process and the active site of extraction. Method: The mice were divided into 9 groups: control group, model group, galactosamine group,bifendate group (150 mg·kg^-1), water extraction group,65% ethanol extract group,95% ethanol extract group of B. longicaule var.giraldii sample No.1 and No.2, delivery 20 mL·kg^-1 by intragastric administration(ig).Model group and control group were given normal saline by ig once a day with the same dose. After continuous administration of 7 days, model of mice were made by intraperitoneal injection of D-galactosamine hydrochloride solution, and after 16 hours, ocular venous blood were collected to determine the serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activities and the malondialdehyde(MDA)content and superoxide dismutase(SOD)activity in liver homogenate. Result: Two origin of B. longicaule var.giraldii on aqueous extraction group, 65% ethanol group and 95% ethanol group showed that they all could decrease the serum ALT and AST activity in model, reduce the content of liver MDA and increase the SOD activity, and the 65% ethanol extract of B. longicaule var.giraldii gathered from East Taibai Mountain had the strongest effect (P<0.01, P<0.05). Conclusion: B. longicaule var.giraldii has obvious protective effect on D-galactosamine hydrochloride induced acute liver injury in mice. There is a dose-effect relationship between its hepatoprotective effect and saikosaponin a, d content.