大孔吸附树脂纯化野菊花多糖工艺

目的:研究大孔吸附树脂对野菊花多糖中所含色素和蛋白质的脱除性能.方法:比较LSA-700B,LSA-21,D101,XDA-8,AB-8,XDA-76种不同型号大孔吸附树脂对野菊花多糖的纯化效果；以脱色率、蛋白质去除率和多糖保留率作为考察指标,探讨温度、多糖质量浓度、pH、转速、流速5个因素对其纯化性能的影响.结果:LSA-21树脂对野菊花多糖的纯化效果较为理想；最佳工艺为温度40℃,多糖质量浓度7 g·L-1,pH 5,转速180 r·min-1,流速3 BV·h-1,径高比1∶8.在此条件下脱色率80.90％,蛋白质去除率52.84％,多糖保留率82.59％.结论:LSA-21大孔吸附树脂对野菊花多糖可以获得较高的纯化效率和多糖保留率.

Purification Technology of Polysaccharide from Chrysanthemum indicum by Macroporous Adsorption Resin

Objective:To study on elution property of pigment and protein contained in polysaccharide from Chrysanthemum indicum.Method:Compared six different types of macroporous resin(LSA-700B,LSA-21,D101,XDA-8,AB-8,XDA-7) for purification effect of polysaccharide from C.indicum by selecting three indicators of decoloration rate,deproteinization rate and retained rate of polysaccharide;and investigated influence of temperature,polysaccharide concentration,pH,rotational speed and elution rate on purification property.Result:Optimum purification conditions of resin LSA-21 were as follows: temperature 40 ℃,polysaccharide concentration 7 g·L-1,pH 5,rotational speed 180 r·min-1 elution rate 3 BV·h-1,diameter/height ratio 1∶8.In these conditions,decoloration rate was up to 80.90%,deproteinization rate was 52.84% and retained rate of polysaccharide was 82.59%.Conclusion:High purification ratio and retained rate of polysaccharide could be obtained by means of purification with LSA-21 macroporus resin.