白芷、没药单煎与合煎对欧前胡素含量及其镇痛作用的影响

目的:比较白芷、没药单煎与合煎对欧前胡素含量及其镇痛作用的影响。方法:采用常规水煎工艺得到白芷单煎液、没药单煎液、白芷-没药合煎液。采用LC-MS测定不同提取液中欧前胡素的含量,乙腈-水(55∶45),流速0.3 min·mL~(-1),进样量5μL,柱温35℃;电喷雾离子源(ESI),正离子扫描模式,单离子监测(SIM)。小鼠分为空白组、阳性组(氨酚双氢可待因片组)和3种水煎液的低、中、高剂量组,阳性药给药剂量为0.24 g·kg~(-1)·d~(-1),白芷单煎液将3,6,12 g·kg~(-1)·d~(-1),没药单煎液将1.5,3,6 g·kg~(-1)·d~(-1),白芷-没药合煎液将4.5,9,18 g·kg~(-1)·d~(-1)设定为低、中、高给药剂量,分别灌胃给药7 d后进行热板试验和扭体试验,记录舔足时间和扭体次数。结果:欧前胡素线性范围0.02~0.20 mg·L~(-1)(R~2=0.991 1),欧前胡素在白芷单煎液中转移率仅0.62%,在白芷-没药合煎液中转移率提高至2.00%。除了个别时间点外,热板试验中单煎液、合煎液各剂量组与空白组比较均有显著性差异(P0.05,P0.01)。扭体试验中白芷单煎低、中剂量组,没药单煎高剂量组和白芷-没药合煎各剂量组与空白组比较均有显著性差异(P0.05,P0.01)。结论:白芷与没药合煎能促进白芷中欧前胡素的溶出。单煎液、合煎液各剂量组均能延长小鼠热板舔足时间,合煎液各剂量组均能延长扭体潜伏时间,合煎液高剂量组对小鼠的疼痛抑制率最高。

Effect of Single and Mixed Decoction of Angelicae Dahuricae Radix and Myrrha on Content of Imperatorin and Their Analgesic Effect

Objective: To compare the effect of single and combined decoction of Angelicae Dahuricae Radix and Myrrha on the content of imperatorin and their analgesic effect.Method: The decoction of Angelicae Dahuricae Radix,Myrrha and their combined decotion were obtained by conventional decoction process.The content of imperatorin in different extract was determined by LC-MS.Mice were divided into blank group,positive drug group(paracetamol and dihydrocodeine tartrate tablets group,0.24 g·kg^-1·d^-1) and low,medium,high dose group of three kinds of decoction,the dose of single decoction of Angelicae Dahuricae Radix was set as 3,6,12 g·kg^-1·d^-1,the dose of single decoction of Myrrha was 1.5,3,6 g·kg^-1·d^-1,the dose of mixed decoction was 4.5,9,18 g·kg^-1·d^-1.Analgesic effect was tested by hot plate test and writhing test in mice after oral administration for 7 d.Result: The linear range of imperatorin was 0.02-0.20 mg·L^-1(R²=0.991 1),the transfer rate of imperatorin was 0.62% in single decoction of Angelicae Dahuricae Radix,but it reached 2.00% in mixed decoction.Except for some time points,there were significant differences between each dose group of single decoction,mixed decoction and the blank group by hot plate method(P<0.05,P<0.01).In the writhing test,there were significant differences between the low,medium dose of Angelicae Dahuricae Radix single decoction,high dose of Myrrha single decoction,each dose group of mixed decoction and the blank group(P<0.05,P<0.01).Conclusion: Mixed decoction of Angelicae Dahuricae Radix and Myrrha can promote the dissolution of imperatorin in Angelicae Dahuricae Radix.Each dose group of single and combined decoction can prolong the licking time of hot plate in mice,and all dose groups of combined decoction can prolong the latent period of torsional body.The high dose group of combined decoction has the highest inhibition rate of pain in mice.