| 消水方联合顺铂对肺癌移植瘤胸腔积液小鼠模型Th17/Treg免疫平衡的影响 |
| |
| 引用本文： | 王兵,关江锋,颜琳琳,蔡煜,赵彪,胡作为,侯炜.消水方联合顺铂对肺癌移植瘤胸腔积液小鼠模型Th17/Treg免疫平衡的影响[J].中国实验方剂学杂志,2018,24(21):131-137. |
| |
| 作者姓名： | 王兵 关江锋 颜琳琳 蔡煜 赵彪 胡作为 侯炜 |
| |
| 作者单位： | 华中科技大学同济医学院附属中西医结合医院武汉市第一医院;武汉科技大学附属汉阳医院;四川省肿瘤医院;中国中医科学院广安门医院 |
| |
| 基金项目： | 国家自然科学基金项目（81241119） |
| |
| 摘 要： | 目的:探讨消水方联合顺铂对肺癌移植瘤胸腔积液小鼠模型Th17/Treg细胞免疫平衡的影响。方法:将75只C57BL/6小鼠随机分为消水方(33.5 g·kg~(-1))组、顺铂(6 mg·kg~(-1))组、消水方(27 g·kg~(-1))+顺铂(6 mg·kg~(-1))组、模型组、空白组。除空白组小鼠胸腔内注射磷酸盐缓冲液(PBS)外,其余各组小鼠均采用Lewis肺癌细胞胸腔注射建立模型,连续给药10 d后各组取10只小鼠处死,采用流式细胞技术检测外周血中Th17,Treg细胞数量;流式微球阵列法(CBA)以及酶联免疫吸附法(ELISA)检测外周血中白细胞介素-6(IL-6),白细胞介素-17A(IL-17A),白细胞介素-21(IL-21),白细胞介素-23(IL-23)和转化生长因子-β1(TGF-β1)水平;实时荧光定量聚合酶链式反应(Real-time PCR),蛋白免疫印迹法(Western blot)检测小鼠脾组织维甲酸相关孤核受体γt(RORγt),叉状头/翅膀状螺旋转录因子(Foxp3)mRNA及蛋白表达。结果:各组小鼠外周血Th17,Treg细胞数量均高于空白组(P0.05,P0.01)。其中消水方+顺铂组、消水方组较模型组能明显增加Th17细胞数量(P0.05),降低Treg细胞数量(P0.01)。消水方+顺铂组IL-6,IL-17A,IL-21含量分别较其余各组均显著升高(P0.01);消水方+顺铂组较消水方组、模型组IL-23含量升高(P0.05),TGF-β1含量降低(P0.05)。与顺铂组、模型组比较,消水方+顺铂组、消水方组可上调脾组织中RORγt mRNA表达(P0.01),下调Foxp3 mRNA表达(P0.05)。与模型组比较,消水方+顺铂组可上调脾组织RORγt蛋白表达(P0.05),下调Foxp3蛋白表达(P0.01)。结论:消水方联合顺铂可能通过上调Th17细胞水平,下调Treg细胞水平,从而纠正Th17/Treg免疫失衡,发挥抑制MPE作用。
|
| 关 键 词： | 肺癌 恶性胸腔积液 消水方 顺铂 辅助性T细胞17 调节性T细胞 |
| 收稿时间： | 2017/12/6 0:00:00 |
Effect of Xiaoshui Formula Combined with Cisplatin on Th17/Treg Immune Balance in Lung Cancer Xenografts in Mice with Pleural Effusion |
| |
| WANG Bing,GUAN Jiang-feng,YAN Lin-lin,CAI Yu,ZHAO Biao,HU Zuo-wei and HOU Wei.Effect of Xiaoshui Formula Combined with Cisplatin on Th17/Treg Immune Balance in Lung Cancer Xenografts in Mice with Pleural Effusion[J].China Journal of Experimental Traditional Medical Formulae,2018,24(21):131-137. |
| |
| Authors: | WANG Bing GUAN Jiang-feng YAN Lin-lin CAI Yu ZHAO Biao HU Zuo-wei and HOU Wei |
| |
| Affiliation: | Integrative Medicine Hospital Affiliated of Tongji Medical College, Huazhong University of Science and Technology, Wuhan No.1 Hospital, Wuhan 430022, China,Integrative Medicine Hospital Affiliated of Tongji Medical College, Huazhong University of Science and Technology, Wuhan No.1 Hospital, Wuhan 430022, China,Hanyang Hospital Affiliated of Wuhan University of Science and Technology, Wuhan 430050, china,Integrative Medicine Hospital Affiliated of Tongji Medical College, Huazhong University of Science and Technology, Wuhan No.1 Hospital, Wuhan 430022, China,Sichuan Cancer Hospital, Chengdu 610041, China,Integrative Medicine Hospital Affiliated of Tongji Medical College, Huazhong University of Science and Technology, Wuhan No.1 Hospital, Wuhan 430022, China and Guang''anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China |
| |
| Abstract: | |
| |
| Keywords: | lung cancer malignant pleural effusion Xiaoshui formula cisplatin Th17 cells Treg cells |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
|
点击此处可从《中国实验方剂学杂志》下载全文 |
|
