杠板归抗二甲基亚硝胺诱导大鼠肝纤维化的作用及其机制研究

目的:研究杠板归对二甲基亚硝胺(DMN)诱导的大鼠肝纤维化的作用及其机制.方法:80只SD大鼠随机分为正常组、模型组、杠板归高、中、低剂量组和秋水仙碱组共6组.除正常对照组以外,其余各组大鼠均以0.5％ DMN溶液(1.6mL·kg-1)腹腔注射,每周连续3天,每天1次,第1周用2/3剂量,以后用全量.秋水仙碱组剂量为0.1 mg·kg-1,杠板归高、中、低剂量组分别为7.5,5.0,2.5 g·kg-1,各组给药1次/d,连续4周.4周末摘大鼠眼球取血,酶联免疫吸附法(ELISA)检测血清透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(Ⅳ-C)；苏木精-伊红染色(HE染色)观察肝纤维化(HF)的形成；免疫组化检测转化生长因子β1(TGF-β1).结果:与模型组比较,杠板归高、中剂量组大鼠血清HA,LN,PCⅢ,Ⅳ-C水平均显著低于模型组(P ＜0.05或P＜0.01),免疫组织化学显示杠板归高、中剂量组中大鼠肝脏TGF-β1表达明显降低(P ＜0.05或P＜0.01),低剂量无统计学意义(P＞0.05).结论:杠板归对二甲基亚硝胺诱导的大鼠肝纤维化具有较好的作用,作用机制可能与杠板归通过保肝、降低TGF-β1表达,从而抑制肝纤维化的启动.

Effect and Mechanism of Polygonum perfoliatum on Hepatic Fibrosis Induced by Dimethylnitrosamine in the Rats

Objective:To observe the therapeutic effect of Polygonum perfoliatum in hepatic fibrosis(HF) induceded by dimethylnitrosamine(DMN) in rats.Method: Rats were given the DMN to establish hepatic fibrosis model,and then given different dose of P.perfoliatum.The liver was used to histopathological analysis.And hepatic levels of transforming growth factor-beta 1(TGF-β1),serum hyaluronic acid(HA)、laminin(LN),procollagen type Ⅲ(PCⅢ),type Ⅳ collagen(Ⅳ-C) were also measured.Result: High and middle dose of P.perfoliatum reduced the content of serum HA,LN,PCⅢ and Ⅳ-C(P<0.05 or P<0.01)and TGF-β1 level in liver(P<0.05 or P<0.01),and the low dose had no effect.Conclusion: The P.perfoliatum attenuates progression of hepatic fibrosis induced by DME in rats.The mechanism is possibly related to suppressing hepatic fibrosis through protecting liver and suppressing the expression of TGF-β1.