丹龙定喘汤对哮喘小鼠气道重塑及MMP-9, TIMP-1的影响

目的:研究丹龙定喘汤对哮喘小鼠气道重塑和基质金属蛋白酶-9(MMP-9)、金属蛋白酶组织抑制因子(TIMP-1)的影响.方法:SPF级BALB/c小鼠56只随机分为正常组、模型组、地塞米松组、丹龙定喘汤组.卵蛋白(OVA)致敏激发小鼠制作哮喘气道重塑模型,在实验第41～69天,正常组、模型组以生理盐水灌胃,其余两组分别给予0.02％地塞米松雾化及ig生药丹龙定喘汤42 g·kg-1治疗.实验结束后HE染色观察哮喘小鼠肺组织病理形态变化,采用ELISA法检测血清MMP-9,TIMP-1水平.结果:丹龙定喘汤能改善哮喘小鼠气道炎性细胞的浸润及支气管平滑肌的增厚;与正常组比较模型小鼠MMP-9,TIMP-1均明显升高(P ＜0.05,P＜0.01);与模型组比较,西药组和丹龙定喘汤组MMP-9,TIMP-1水平明显降低(P＜0.01),MMP-9/T1MP-1比值在两组明显增高(P＜0.05),且两组间未见差异.结论:丹龙定喘汤能够抑制哮喘小鼠血清MMP-9,TIMP-1的分泌,改善哮喘小鼠气道重塑状态.

Effect of Danlong Dingchuan Decoction on Expressions of MMP-9, TIMP-1 and Airway Remodeling in Asthmatic Mice

Objective: To Study the effect of Danlong Dingchuan decoction(DDD) on expressions of matrix metalloproteinases-9(MMP-9), tissue inhibitor-1 of metalloproteinase(TIMP-1) and airway remodeling in asthmatic mice. Method: Fifty six SPF BALB/c mice were randomly divided into normal group, model group, western medicine group and DDD group. The three groups except normal group were induced as asthmatic model in allergized mice with ovalbumin(OVA). During the 41-69 days in the experiment, the normal and model group were treated with normal saline. Meanwhile, the western medicine group and DDD group were respectivelytreated with dexamethasone and DDD. At the end of experiment, we observed the pathomorphological changes in lung issues and took the sample blood to detect MMP-9 and TIMP-1 using ELISA. Result: The pathological changes in lung tissue showed that DDD can significantly reduce the infiltration of inflammatory cells and inhibit bronchial smooth muscle thicken.The level of MMP-9 were significantly increased except the normal group(P<0.05),and TIMP-1 in lung tissue were increased in model group(P<0.01). In western medicine group and DDD group, the content of MMP-9 and TIMP-1 were significantly decreased(P<0.01),but the relative value of MMP-9 and TIMP-1 in the two groups were significantly increased(P<0.05). Conclusion: DDD can inhibit the secretion of MMP-9 and TIMP-1 which suppress airway remodeling in asthmatic mice.