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| 固相萃取-高效液相色谱法测定大黄酸血药浓度 及在大鼠体内的药动学规律 | |
| 引用本文： | 冯素香,谢新年,李建生,屈凌波,梁生旺,王淑美.固相萃取-高效液相色谱法测定大黄酸血药浓度 及在大鼠体内的药动学规律[J].中国实验方剂学杂志,2011,17(18):140-143. |
| 作者姓名： | 冯素香 谢新年 李建生 屈凌波 梁生旺 王淑美 |
| 作者单位： | 1. 河南中医学院,郑州 450008;郑州大学,郑州 450001 2. 河南中医学院,郑州,450008 3. 郑州大学,郑州,450001 4. 广东药学院,广州,510006 |
| 基金项目： | 河南省高校青年骨干教师资助项目(2009GGJS-065);国家自然科学基金资助项目(81073024);郑州市科技攻关项目(0910SGYS33390-7) |
| 摘 要： | 目的:建立测定大鼠血浆中大黄酸的固相萃取-高效液相色谱方法,研究大黄酸在大鼠体内的药动学规律。方法:单次给予SD大鼠不同剂量的大黄酸,于不同时间点采集大鼠血浆。以固相萃取法处理血浆样品,用HPLC内标法测定血药浓度,色谱柱为Venusil XBP C18(L)(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%磷酸水(75∶25),柱温为30℃,检测波长254 nm,以DAS2.0软件拟合计算药动学参数。结果:大黄酸的血药浓度在0.019 2～11.72 mg.L-1线性关系良好(R2=0.999 1),检测限为0.009 6 mg.L-1,定量限为0.019 2 mg.L-1,提取回收率均大于80%,日内、日间精密度RSD均小于6%。结论:大鼠灌胃给药大黄酸血药浓度-时间曲线呈二室模型。该法操作简便、快速、灵敏,适用于大黄酸在大鼠体内的药物动力学研究。 |
| 关 键 词： | 大黄酸 药动学 高效液相色谱法 固相萃取 |
| 收稿时间： | 4/4/2011 12:00:00 AM |
Determination of Rhein in Plasma by SPE-HPLC and Its Pharmacokinetic Study in Rats |
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| FENG Su-xiang,XIE Xin-nian,LI Jian-sheng,QU Ling-bo,LIANG Sheng-wang and WANG Shu-mei.Determination of Rhein in Plasma by SPE-HPLC and Its Pharmacokinetic Study in Rats[J].China Journal of Experimental Traditional Medical Formulae,2011,17(18):140-143. | |
| Authors: | FENG Su-xiang XIE Xin-nian LI Jian-sheng QU Ling-bo LIANG Sheng-wang and WANG Shu-mei |
| Affiliation: | Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China; Zhenzhou University, Zhengzhou 450001, China; Guangdong College of Pharmacy, Guangzhou, 510006, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Zhenzhou University, Zhengzhou 450001, China;Guangdong College of Pharmacy, Guangzhou, 510006, China;Guangdong College of Pharmacy, Guangzhou, 510006, China |
| Abstract: | Objective:To develop a simple HPLC method for the determination of auraptene in rats plasma,and to investigate the pharmacokinetics of auraptene after intravenous injection in rats.Method:The contents of auraptene in rats plasma were determined by RP-HPLC.The software DAS 2.1 was used to calculate the pharmacokinetic parameters.Result:After intravenous injection to rats,direct proportion was found between AUC and the doses,and linear relationship was observed between Cmax and the doses.MRT did not extend wi... |
| Keywords: | rhein pharmacokinetics HPLC SPE |
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