川芎嗪和葛根素配伍对脑缺血损伤大鼠 神经功能及脑组织形态学变化的影响

目的:通过观察大鼠局灶性脑缺血再灌注后脑功能行为及脑组织形态学改变,研究川芎嗪和葛根素配伍对脑缺血再灌注损伤的保护作用机制。方法:采用改良的Pulsinelli四血管法建立大鼠脑缺血损伤模型,将SD大鼠随机分为假手术组、模型组、川加葛组(川芎嗪50 mg.kg-1和葛根素50 mg.kg-1)、尼莫地平组(0.5 mg.kg-1),于再灌0,6 h ip。用药后,观察药物对脑缺血引起的神经功能缺损症状、脑组织形态学变化的影响。结果:造模后大鼠大脑皮质脑组织形态学结构发生改变及神经元损害等改变。川芎嗪与葛根素配伍不仅能减轻模型大鼠的神经功能缺损症状,且大鼠大脑皮质神经元病变有一定程度的改善。结论:川芎嗪和葛根素配伍对脑缺血损伤有一定防治作用。

Protective Effects of Ligustrazine Combined with Puerarin on Neurological Deficits and Morphological Changes in Cerebral Ischemia Injury in Rats

Objective: To investigate the protective effects of Ligustrazine combined with puerarin on the changes of neurological deficits. Method: Rats were randomized into sham-operation group, model group, Ligustrazine-puerarin group(ligustrazine 50 mg·kg^-1-puerarin 50 mg·kg^-1)and nimodipine group(0.5 mg·kg^-1). The model was established by 4 vessel occlusion method. The changes in nerve function and brain morphology were evaluated. Result: After ischemia reperfusion, the model group neurologic deficit and morphological changes were higher than that in the sham-operation group significantly (P<0.01).Neuronal damage and inflammatory reactions could be found in the model group(the sections stained with Nissle staining). In Ligustrazine-puerarin group and nimodipine group, the neurologic deficit scores and morphological changes were lower than that in the model group (P<0.05, P <0.01). Conclusion: Ligustrazine-puerarin can prevent and alleviate the ischemia and reperfusion injury to some degree.